摘要
利用玉米表达序列标签数据库(dbEST)电子克隆了玉米基因PIS2的cDNA全长,并采用反转录聚合酶链反应(RT-PCR)技术得到了包含该基因完整开放阅读框(ORF)的cDNA序列;借助水稻非冗余核酸数据库联合应用电子克隆技术和RT-PCR技术克隆得到玉米主易化子超家族蛋白基因MFSP的cDNA全长,并进一步利用植物基因组数据库(PlantGDB)快速地获得了该基因的基因组全长序列。研究证实,充分利用分子生物信息数据库,可以简便地克隆得到玉米目的基因的cDNA乃至基因组全长序列。
A cDNA containing the complete opening reading frame （ORF） of the putative PIS2 （phosphatidylinositol synthase 2） gene was cloned from maize （ Zea may L. ） by means of the RT-PCR method coupled with the in silico cloning ap- proach based on the expressed sequence tags （EST） database （dbEST）. Full-length cDNA of the maize MFSP （major facilitator superfamilyprotein） gene was isolated by the RT-PCR method combined with the in silico cloning with assis- tance of the Oryza sativa non-rexlundant nucleotide database. And by using the plant genome database （PlantGDB）, the genomic sequence of the MFSP gene was obtained. These results of the study indicate that full-length cDNA and genomic sequences of genes can be easily obtained by making the best use of molecular bioinformatics databases.
出处
《高技术通讯》
EI
CAS
CSCD
北大核心
2009年第6期656-660,共5页
Chinese High Technology Letters
基金
863计划(2006AA10A107)资助项目
