摘要
目的观察佐剂关节炎(AA)大鼠红细胞参数(红细胞计数RBC、血红蛋白含量HGB、网织红细胞计数RET#、平均红细胞体积MCV)、血清细胞因子(IL-1β、IL-10)。方法将16只清洁级W istar雄性大鼠随机分为正常对照组、模型对照组。每组8只,用弗氏完全佐剂(CFA)0.1 ml分别向除正常对照组以外的其余大鼠右后足跖皮内注射诱发大鼠产生关节炎,观察两组大鼠红细胞参数(红细胞计数RBC、血红蛋白含量HGB、网织红细胞计数RET#、平均红细胞体积MCV)和血清细胞因子(IL-1β、IL-10)的变化。结果(1)与正常对照组相比,模型对照组大鼠IL-1β显著升高,而RBC、HGB、MCV、IL-10的值降低(P<0.05或P<0.01)。(2)AA大鼠的RBC、MCV、HGB与IL-1β、足肿胀度、关节炎指数呈负相关,与IL-10呈正相关。RET#两组无明显差异(P>0.05)。结论炎症与佐剂关节炎大鼠RBC、MCV、HGB下降与疾病活动有关。
Objective To observe the red cell parameters （The red cell counts RBC, hemoglobin content HGB, reticulocyte count RET#,mean corpuscular volume MCV）, serum cytokines （IL-1β, IL-10） in adjuvant arthritis rats and do the research of the correlation. Methods 16 SD-masculine rats were randomly divided into normal control group and model group, which both had 8 masculine rats. Model group was intracutaneously injected with 0. 1 ml Freund＇s complete adjuvant in the right hindlimb. To observe the changes of red cell parametes （ RBC, HGB, RET #, MCV） and serum cytokines （IL-1β, IL-10） in two groups. Results （1）Compared with the normal control group, the level of serum IL-1β in the model control group remarkablely went up,but the values of RBC, HGB, MCV, IL-10 decreased. （P 〈 0.05 or P 〈 0. 01 ） （2）The level of RBC, MCV, HGB in AA rats was found in negative correlation to the IL-1β,degree of foot swelling and index of arthritis, but postive correlation to the level of IL-10, RET# had no obvious difference between two groups. Conclusion The inflammation is related to the disease activity and reduction of RBC,MCV and HGB in adjuvant arthritis rats.
出处
《中国临床保健杂志》
CAS
2009年第2期146-148,共3页
Chinese Journal of Clinical Healthcare
基金
国家中医药管理局科研课题专项基金(04-05LP27)
安徽省十一五攻关项目(07010300204)
安徽科技重点研究项目(06023068)
