摘要
目的观察新风胶囊对佐剂性关节炎(Adjuvant arthritis,AA)大鼠肺部高分辨率CT(High resolution ratio CT,HRCT)改变及血清细胞因子的影响。方法将60只大鼠随机分为正常对照组、模型对照组、甲氨喋呤(Methotrexate,MTX)组、雷公藤多苷片(Tripterygium wilfordii polycoride tablet,TPT)组和新风胶囊(Xinfeng capsule,XFC)组,每组12只,分别向除正常对照组以外的其余动物右后足跖皮内注射弗氏完全佐剂0.1ml致炎,致炎后第19天开始给药。正常对照组及模型对照组均给予生理盐水,其余3组分别给予MTX、TPT、XFC。给药1个月后,观察各组大鼠肺部HRCT及血清细胞因子的变化。结果HRCT表现为模型对照组大鼠肺轮廓欠清晰,肺部血管影扩张,边缘模糊且不规则,两肺有索条状密度增高影,邻近胸膜轻度肥厚;甲氨喋呤组大鼠肺部可见沿着肺纹理分布的斑片状密度增高影,肺血管纹理模糊,边缘不清楚;雷公藤多苷片组大鼠两肺可见多发性结节状及斑片状密度增高影,境界欠清晰;新风胶囊组大鼠肺轮廓清晰,肺纹理分布均匀,部分肺组织显示斑点状密度增高影。与正常对照组比较,模型对照组TNF-α显著升高(P<0.05);与模型对照组比较,新风胶囊组TNF-α显著降低(P<0.01),而与其余2治疗组无显著差异(P>0.05);与正常对照组比较,模型对照组IL-10显著降低(P<0.01);与模型对照组比较,新风胶囊组IL-10显著升高(P<0.01),而与其余2治疗组无显著差异(P>0.05)。结论新风胶囊能下调致炎因子TNF-α、上调抗炎因子IL-10、抑制致炎效应、增强抗炎效应,在降低AA大鼠足跖肿胀度的同时,也能改善肺部影像学改变。
Objective：To observe the influence of Xinfeng capsule（XFC） on pulmonary HRCT images and cytokines（ TNF-α, IL-10） in blood serum adjuvant arthritis rats. Methods ：60 rats were averagely divided into normal control group, model control group, methotrexate（MTX） treated group,tripterygium wilfordii polycoride tablet （TPT） treated group, XFC treated group, 12 in each. Except for the rats of normal group,the others were intracutaneously injected with 0. 1 ml of Freund＇ s complete adjuvant in the right hindlimb. After 19 days ,different drugs were given to all rats. Pulmonary HRCT images were obtained and the blood samples were reserved for assaying eytokines. Results：In model control group ,the lungs＇ outlines weren＇t clear and the blood vessels expanded with the edges of them being obscure and unregular. There were high density images shaped as rope and slip in lungs of model control group rats and the pleura near the images were a little thickening. In MTX treated group, there were high density images shaped as slice along pulmonary veins. The veins of the blood vessels were obscure and the edges were unclear. In TPT treated group,there were multiple high density images shaped as tubercle and slice and the boundary were not clear. In XFC treated group, the lungs＇ outlines were clear and the veins were well-distributed. High density images shaped as spot were found in part lungs. Compared with normal control group, tumor necrosis factor-α（TNF-α） in blood serum ascended obviously in model control group（ P 〈 0. 05 ）, but interleukin-10（IL-10） lowered obviously（ P 〈 0. 01 ）. TNF-α of XFC treated group were lower than that of model control group（ P 〈 0. 01 ） and IL-10 of XFC treated group were higher than that of model control group （ P 〈 0.01 ）, but there were no obvious differences among the three treated groups （ P 〉0. 05）. Condusion：XFC eliminates swelling by down-regulating the levels of TNF-α, up-regulating the levels of IL-10, restraining the inflammatory reaction. In adittion ,cytokines participated in forming pulmanory fibrofication. So the changes of pulmanory HRCT images were also improved at the same time.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2007年第3期243-246,250,共5页
Chinese Journal of Immunology
基金
国家中医药管理局专项课题(04-05LP27)
安徽省自然科学基金项目(050431002)资助
