摘要
目的：了解动脉平滑肌细胞（ＡＳＭＣ）的蛋白激酶Ｃ（ＰＫＣ）含量及亚型分布，以及与细胞增殖的关系。方法：采用点印迹技术及ＭＴＴ方法，检测了人ＡＳＭＣ颗粒部分与胞浆部分ＰＫＣ的６种亚型的含量和分布。结果：①人ＡＳＭＣ细胞中主要有ＰＫＣα、ＰＫＣβ、ＰＫＣζ３种亚型分布，并发现６３％分布在胞浆中；②人ＡＳＭＣ细胞ＰＫＣ总量随着佛波酯（ＰＭＡ）、胰岛素浓度的增加而增加，人ＡＳＭＣ细胞增加数量也随着ＰＭＡ、胰岛素的浓度增加而增加。结论：人ＡＳＭＣ细胞中以ＰＫＣα、ＰＫＣβ、ＰＫＣζ３型含量较多，胰岛素可能是通过激活ＰＫＣ信号传导途径引起细胞的基因表达增高，导致ＡＳＭＣ细胞增殖。
Objective: To investigate the effects of insulin on distribution and content of protein kinaseC isoforms (PKCIs) in human arterial smooth muscle cells (ASMCs). Methods: Dot blot hybridization and MTT microenzymatic determination were used to detect the distribution of six subtypes of PKCIs and then to explore the relationship between the changes of subtypes and the proliferation of human ASMCs after the interference of insulin. Results: ①human ASMCs mainly contained PKCI subtypes alpha, beta and epsilon and 63% of them were distributed in cytoplasm; ②The total PKCIs in human ASMCs and ASMC count were increased along with the increase of concentration of insulin and PMA. Conclusion: Most of the PKCIs in ASMCs belong to subtypes alpha, beta and epsilon. Insulin induces the proliferation of ASMCs through activating the PKCI signal pathway.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1998年第2期118-120,共3页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
平滑肌
胰岛素
蛋白激酶C亚型
高血压
arterial smooth muscle cell
insulin
protein kinaseC isoform
subtype
