摘要
目的观察榄香烯和三苯氧胺对乳腺癌细胞的抑制效应,尤其是两者联合应用有无协同增效作用。方法将MCF-7细胞培养于RPMI1640培养基中,置入37℃,100%湿度,5%(体积分数)的CO2培养箱内。接种细胞并加入实验药物,用CCK-8法检测三苯氧胺联合榄香烯对细胞的增殖抑制效应,用酶标仪在450nm测量每孔吸光度值,计算每组细胞抑制率。结果无毒剂量的榄香烯与治疗剂量的三苯氧胺联合应用,吸光度均值较单用三苯氧胺明显下降,并且相应的细胞抑制效应较单用组明显上升。结论无毒剂量的榄香烯能够使三苯氧胺对乳腺癌细胞的抑制效应显著增强,提示榄香烯与三苯氧胺联合应用具有协同作用。
Objective To explore if clement can syncrgize tamoxifen through using non-cytotoxic dose of clement and tamoxifen in combination on MCF-7cell line of breast cancer. Methods First, MCF-7 cell line was cultured in RPMI1640. Then, TAM＇s inhibitory effect united with clement on MCF-7 cell line was analyzed by using CCK-8 test. Results When adding non-cytotoxic dose of clement and curative dose of tamoxifen into the two experimental groups, the absorptivity in the two groups reduced notably, compared with that of the groups using tamoxifen alone; accordingly, the inhibition rate in two mixing groups increased obviously compared with that in single-drug groups. Conclusion Element of innocuous dose can remarkably enhance the inhibitory effect of tamoxifen, which indicates that clement may synergize the effect of tamoxifen on breast cancer.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期74-77,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
陕西省自然科学基金资助项目(No.20021210-G3(05))
关键词
乳腺癌
三苯氧胺
榄香烯
协同作用
breast cancer
tamoxifen
elemene
synergistic effect
