摘要
采用热启动PCR结合RFLP的方法分析90例冠心病患者和120名健康对照者载脂蛋白B基因第26外显子Msp1位点的多态性,产生3种基因型:M_(1)M_(1)、M_(1)M_(2)、M_(2)M_(2)。冠心病组等位基因M_(1)、M_(2)频率分别是0.917,0.083;对照组M_(1)、M_(2)频率分别为0.975,0.025,基因频率在两组间差异有显著性意义。少见等位基因M_(2)在冠心病组较多见。但各基因型之间血脂、脂蛋白、载脂蛋白在两组中差异均无显著性。这种基因多态性是否影响ApoB与LDL受体的结合,从而促进冠心病、高脂血症等疾病的发展,尚有待进一步研究探讨。
By the hotstart PCRRFLP we analyzed Msp1 RFLP of exon No.26 of human apolipoprotein B(ApoB)gene in 90 patients with CAD and 120 of the control group.Three genotypes were examined:M_(1)M_(1),M_(1)M_(2)and M_(2)M_(2).The distributions of ApoB gene frequencies were 0.917 for allele M_(1),0.083 for rare allele M_(2)in CAD group,and 0.975 for M_(1),0.025 for M_(2)in the control.The gene frequency of allele M_(2)was more in CAD group.However,no significant differences in lipids,lipoproteins and apolipoproteins of every genotype were observed in the patients or in the control.The mutation may lead to an abnormal binding of ApoB to LDLreceptor associated with atherosclerosis and hypercholestrolemia,a possibility remains to be explored.
作者
徐琼
洪嘉玲
周新
Xu Qiong;Hong Jiaing;Zhou Xin(Laboratory of Medicine,Second Affiliated Hosptical,Hubei Medical University,Wuhan 430071,China)
关键词
载脂蛋白B
遗传学
基因多态性
冠心病
MeSH apolipoprotein B/GE
genetic polymorphism
gene frequency
