摘要
目的研究我国藏族人群载脂蛋白Ｂ（ａｐｏＢ）基因３′端高可变区等位基因的多态性及其与血脂水平的关系。方法采用聚合酶链反应和聚丙烯酰胺梯度凝胶电泳方法分析ＰＣＲ产物。结果从９９份ＤＮＡ样品中分离到２０种等位基因，其频率分布呈双峰型（Ｂｉｍｏｄｅｌ）；以ＨＶＥ３２和ＨＶＥ３４最为常见。结论藏族人群ａｐｏＢ基因３′高可变区（ＶＮＴＲ）等位基因多态性既与重复单位的拷贝数不同有关，也与每一拷贝内的核苷酸排列顺序差异有关，大等位基因比例较低，与我国蒙族近似。
Objectives To study the distribution and frequency of apoB gene 3 end alleles and try to find if there is any association with the level of plasma lipids Methods PCR and gradient polyacrylamide gel electrophoresis were used to analysize the chromsome DNA and PCR products Results Totally 20 alleles were found in the Tibetan group and the distribution of alleles was bimode The HVE32 and HVE34 were most common, and the frequencies of HVE32 and HVE34 were 027 and 021 respectively Conclusion Polymorphism apoB gene resides not only in the variable numbers of the repeat unit, but also in the variation in the oligonucleotide sequence within the repeat units as well as the variation in the arrangement of the repeat units
出处
《中华医学杂志》
CAS
CSCD
北大核心
1999年第7期505-508,共4页
National Medical Journal of China
基金
国家攀登计划基金
国家自然科学基金
