摘要
应用聚合酶链反应研究了１０３例冠心病人和１００例正常人ａｐｏＢ基因３＇端可变数目串联重复序列的多态性。在２０３例受检者中共检出１２种等位基因（３＇β２９─５１）。其中以３＇β３７等位基因最为常见，其次为３＇β３９。等位基因分布的主要形式与来自不同种族的报告基本相似，但等位基因相对频率分布有种族差异。冠心病组中串联重复拷贝数目≥３９的等位基因（３＇ＶＮＴＲ─Ｂ）频率明显高于正常对照组（Ｐ＜０．００１）。同样，冠心病组中３＇β４３以上的等位基因也较正常对照组明显增多（Ｐ＜０．０１）。此外，３＇ＶＮＴＲ－Ｂ等位基因还与血浆ＴＣ、ＬＤＬ─Ｃ水平升高，ＨＤＬ─Ｃ水平降低有关。提示，ａｐｏＢ基因ＶＮＴＲ多态性可能在一定程度上参与冠心病的发生和发展过程。
The polymorphisms of variable number of tandem repeats(VNTR)3'to theapolipoprotein B)gene were investigated using polymerase chain,eaction(PCR)in 103 patients with documented coronary heart diseas. (CHD)and 100 healthy individualsselected from Chinese Han nationality.Twelve segregating alleles(3'29─51)were observedin 203 subjects.The most common allele was 3'β37,followed by 3'β39 with frequencies of0.362 and 0.296,respectively;This mode of allele distribution was coincided with,butthe relative frequencies of alleles was differed from the results of different ethnicgroups.In comparison of the allele frequencies between patients and controls,allelesbigger than 3'β39(3VNTR─B)were much more common among patients than in the controls(P<0.001).The same trend of frequencies of the alleles bigger than 3'β43 between thetwo groups was also found(P< 0.01).Moreover,in CHD group patients with plasmalevels of TC > 3.88mmnol/L,LDL─C>2.59mmol/L and HDL─C<1.16mmol/L had muchhigher frequencies of 3'VNTR─Ballele(P< 0.01).Therefore,it was suggested that3'VNTR─B allele might be involved in the development of coronary atherosclerosis,presumably through their influenees on lipid metabolism.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1996年第1期4-9,共6页
Chinese Journal of Pathophysiology
基金
卫生部"八
五"攻关基金
关键词
冠心病
载脂蛋白B
基因
多态性
Coronary disease· Apolipoprotein B· Genes·Polymorphism LDL─C=low density lipoprotein─choIesterol,HDL─C=high density lipoprotein─cholesterol
TC=Total cholesterol
