摘要
采用邻苯三酚自氧化法测定山葡萄籽和皮中原花青素SOD-L的活性为192.85U/g和78.45U/g。通过实验确定了适宜的测定条件:测定波长325nm,缓冲体系为Tris-HAc缓冲液,浓度为50mmol,pH8.2。原花色素SOD-L对连苯三酚自氧化的抑制率达到91%。
Pyrogallol autooxidation method was applied to determine SOD-like activities of proanthocyanidins in seeds and peels of Vitaceae amurensis. The results indicated that the SOD-like activities of proanthocyanidins in seeds and peels of Vitaceae amurensis are 192.85 U/g and 78.45 U/g, respectively. The optimum parameters for this assay are as follows： determining absorbance at the wavelength of 325 nm, and taking Tris-HA at pH 8.2 and at the concentration of 50 mmol/L as buffer system. The inhibition percentage of proanthocyanidins against pyrogallol antooxidation reaches 91%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第7期72-74,共3页
Food Science
基金
鞍山市科委科学研究项目(2007SF26)
