摘要
本研究观察了脑出血后SOMmRNA在大脑皮层表达的变化以及中药清开灵注射液对其表达的影响。Wistar大鼠用胶原酶脑内注射法诱发脑出血，动物分别存活1、3、7d后，用地高辛标记的SOMcRNA探针进行杂交，抗地高辛抗体孵育，NBT／BCIP显色，用图像分析仪测量前额皮层Ⅱ～Ⅲ层的SOMmRNA阳性神经元。结果表明：大鼠脑出血后大脑皮层Ⅱ～Ⅲ层内表达SOMmRNA的神经元数目逐步减少，单个细胞SOMmRNA的光密度也逐步降低；清开灵可增加SOMmRNA表达的数量和神经元内SOMmRNA的强度。SOM在中枢神经系统中分布广泛，功能复杂。目前认为它还是一种神经营养性物质。我们认为SOMmRNA在脑出血后表达减少是神经元受损的表现，而清开灵治疗后SOMmRNA表达的上调是其治疗机理的重要部分。
It is now known that somatos ta tin (SOM ) is involved in certain kinds of central nervous system diseases. In this paper, we used in situ hybridization histochemistry to observe the changes of the expression of SOM mRNA in cerebral cortex after intracerebral hemorrhage in rats. The effects of a traditional Chinese medicine Qin Kai Ling(QKL)were also investigated. The number of the neurons which expressed SOM mRNA in layerⅡ and Ⅲ of cerebral cortex was decreased gradually after intracerebral hemorrhage and the positive intensity of single neuron was also decreased while the QKL inhibited the decrease. These data suggest that the function of the neurons in cerebral cortex is damaged after intracerebral hem0rrhage and traditional medicine may have some POsitive effects by regulating the expression of SOM mRNAs.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
1998年第1期61-64,共4页
Chinese Journal of Neuroanatomy
关键词
脑出血
生物抑素
MRNA
清开灵
前额皮层
intracerebral hemorrhage, SOM mRNA, Qing Kai Ling, in situ hybridization histochemistry
