摘要
通过构建一套整合载体，将来自嗜热脂肪芽孢杆菌的耐高温α－淀粉酶基因以及质粒ｐＣ１９４上的一段Ｃｍ抗性基因随机整合至枯草杆菌１Ａ２８９染色体上，通过提高整合菌的抗氯霉素能力和反复进行整合，可提高耐高温α－淀粉酶基因在染色体上的拷贝数，最终耐高温α－淀粉酶基因在染色体上的拷贝数由整合初的一个增至七个以上。同时，在无选择压力的情况下。
By constructing a set of integration vectors,the thermostable α amylase gene from B.stearothermophilus and Cm resistance gene from pC194 were randomly integrated into the genome of B.subtilis 1A289.The number of the integrated thermostable α amylase gene copies was further increased by repeating the methed of random integration or by amplifying the gene using Cm selection,The copy number of the thermostable α amylase gene in genome increased from one to seven.Under the optimal conditions,the thermostable α amylase activity reached 220 u/ml without selection pressure.
出处
《工业微生物》
CAS
CSCD
1998年第1期7-12,共6页
Industrial Microbiology
