摘要
采用测定趋化性的方法，观察了表皮生长因子（ＥＧＦ）和纤维连接蛋白（ＦＮ）对肺泡巨噬细胞（ＡＭ）趋化运动的影响。结果显示，ＥＧＦ能抑制ＡＭ的趋化性，量效关系显著（ｒ＝－０．９９１０，Ｐ＜０．０１），而ＥＧＦ本身对ＡＭ并不表现趋化吸引作用。ＦＮ亦能抑制ＡＭ的趋化性（Ｐ＜０．０１），ＥＧＦ和ＦＮ两者协同作用时，对ＡＭ趋化性的抑制程度大于各自的单独作用（Ｐ＜０．０１）。实验证明ＥＧＦ具有调节ＡＭ趋化运动的非促丝裂功能。提示肺内的细胞因子ＥＧＦ和细胞外基质成分ＦＮ参与肺部炎症及免疫反应的调控。
? To evaluate the hypothesis that epidermal growth factor (EGF) may play a role in modulating the function of alveolar macrophage(AM),the chemotaxis of AM was examined using the technique of blindwell chamber.It was observed that the chemotaxis of rat AM was inhibited by EGF in a dose-dependent manner (r=-0.9910,P<0.01),but EGF did not show any chemoattractant effect.The AM chemotaxis could also be reduced by fibronectin(P<0.01).The down-regulation level of co-existence with EGF and fibronectin on AM chemotaxis was significantly lower than that of EGF or fibronectin alone(P<0.01).These studies demonstrate the existence of a non-mitogenic function of EGF on the AM migration.It is suggested that EGF and fibronectin might take part in modulating the inflammation and immunity response in lung.
出处
《中国应用生理学杂志》
CAS
CSCD
1998年第1期43-45,共3页
Chinese Journal of Applied Physiology
关键词
表皮生长因子
肺泡
巨噬细胞
趋化性
epidermal growth factor
alveolar macrophage
fibronectin
chemotaxis
