摘要
用放射免疫法检测支气管上皮细胞（ＢＥＣ）和肺泡巨噬细胞（ＡＭ）血管活性肠肽（ＶＩＰ）的分泌，放射配基法测定ＢＥＣ的ＶＩＰ受体（ＶＩＰＲ）表达，观察表皮生长因子（ＥＧＦ）的调控效应。结果示：①电镜下，ＢＥＣ细胞内有神经肽分泌颗粒特征，ＢＥＣ及ＡＭ均有ＶＩＰ基础分泌；②ＥＧＦ呈剂量依赖性促进ＢＥＣ的ＶＩＰ分泌，并促进ＡＭ分泌ＶＩＰ；③ＥＧＦ呈剂量依赖性上调ＢＥＣ的ＶＩＰＲ表达。提示气道上皮存在ＶＩＰ的自分泌或旁分泌，其保护效应受局部生长因子的调控。
In order to confirm the essential role of vasoactive invtestinal peptide(VIP) on protection of local airway epithelium and investigate the modulation of the protective effect of VIP, secretion of VIP and expression of VIP receptor(VIPR) were studied in bronchial epithelial cells(BEC), and alveolar macrophages(AM). The radioimmunoassay was used for determining VIP secretion and the radio ligand affinity analysis for VIPR expression. The effect of epidermal growth factor(EGF) was observed. The results showed that: ①Both BEC and AM secreted VIP basically without remarkable stimulus, and some vesicles with electron dense core which usually exist specifically in neuropeptide secreting cells were found in plasma of BEC; ②In a dose dependent manner, EGF promoted BEC to secrete VIP( r =0.84, P <0.05), and it was found that VIP secretion from AM was also elevated in EGF pretreated group( P <0.05); ③EGF exhibited a dose dependent up regulatory effect on the expression of VIPR in BEC( r =0.95, P <0.01). These results indicate that VIP may play an essential role in the protection at local airway by autocrine or paracrine, and the protective effect can be modulated by growth factors.
出处
《湖南医科大学学报》
CSCD
1999年第2期99-102,共4页
Bulletin of Hunan Medical University
基金
国家自然科学基金
关键词
表皮生长因子
上皮细胞
支气管
血管活性肠肽
epidermal growth factor
epithelial cells
bronchial
vasoactive intestinal peptide
