摘要
[目的]为了探索水稻最佳SSR-PCR反应体系。[方法]以水稻R117为试验材料,研究Mg2+、dNTP、引物浓度、Taq DNA聚合酶用量以及退火温度对水稻SSR-PCR扩增结果的影响。[结果]当Mg2+浓度2.00 mmol/L,dNTP浓度0.15 mmol/L,引物浓度0.30μmol/L,Taq DNA聚合酶用量2.0 U,退火温度56.6℃时,PCR扩增DNA条带最亮。[结论]在20μl反应体系中,上述结果为水稻最适SSR-PCR反应体系。
[ Objective ] The SSR-PCR system of rice（ Oryza sativa L. ） was explored. [ Method ] The rice variety R117 being taken as material, the effect of Mg^2＋ , dNTP, primer, Taq NDA polymerase and temperature on SSR-PCR amplification reaction was studied. [ Results] The result showed that the optimal SSR-PCR reaction system in rice included Mg^2＋, 2. 00 mmoL/L; dNTP, 0.15mmol/L; primer, 0.30 μmol/L; Taq DNA polymerase, 2.0 U and annealing temperature, 56.6 ℃, which resulted in the best PCR amplification band. [ Conclusion] In the reaction system, the condition would produce the best result.
出处
《安徽农业科学》
CAS
北大核心
2009年第8期3441-3443,3447,共4页
Journal of Anhui Agricultural Sciences
基金
温州市科技计划项目(N2006A001)
