摘要
目的探讨整合蛋白α５亚基与原发性肝癌的关系。方法应用免疫组化技术（ＡＢＣ法）和Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交检测整合蛋白α５亚基在原发性肝癌中的表达。结果发现在７９例癌与癌周组织α５阳性率分别为３２．９％和８１．０％，两者间差异有显著性（Ｐ＜０．０１）。直径≤５ｃｍ的肝癌α５阳性率高于直径＞１０ｃｍ的肝癌（５５．６％比１０．０％，Ｐ＜０．０１），分化较好的肝癌α５阳性率高于分化不良者（４０．６％比１６．０％，Ｐ＜０．０５），已发生明确肝内转移（包括肝内播散和门静脉癌栓形成）的肝癌α５阳性率低于未发生肝内转移者（２０．６％比４２．２％，Ｐ＜０．０５）。α５亚基表达与患者年龄、血清甲胎蛋白水平、乙型肝炎病毒感染、肝硬化有无等因素均无明显相关（Ｐ＞０．０５）。Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交结果也同时显示，非侵袭性肝癌α５表达高于侵袭性肝癌。结论整合蛋白α５低表达与肝癌增大、分化程度低、侵袭转移发生等恶性表型相关，可能对这些恶性表型起负性调节作用。
Objective To investigate the relation between integrin α 5 subunit and malignant phenotypes of hepatocellular carcinoma. Methods Seventy nine formalin fixed sections and ten blocks of fresh HCC tissue were obtained. The integrin α 5 subunit was measured with immunohistochemistry assay (ABC method) and Northern blot analysis. Results In 79 cases of hepatocellular carcinoma, tumor tissue had lower expression than adjacent non tumor tissue (32.9% vs 81.0%, P <0.01). The α 5 positive rate in small HCC was higher than large HCC (55.6% vs 10.0%, P <0.05). HCC with good differentation and capsulation and without intrahepatic metastasis expressed higher α 5 than those with poor differentiation, no capsulation and intrahepatic metastasis. Similar result is also confirmed by Northern blot analysis. Conclusion Our study indicated that integrin α 5 subunit expression was correlated with the growth, differentation, invasiveness, metastasis and progression of HCC. It was possible that integrin α 5 subunit is a negative regulator of these malignant phenotypes of HCC.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1998年第1期7-9,共3页
Chinese Journal of Internal Medicine
基金
"九五"国家科技攻关和上海市领先专业基金
