摘要
１００ｎｍｏｌ／Ｌ佛波酯（１２－Ｏ－ｔｅｔｒａｄｅｃａｎｏｙｌｐｈｏｂｏｌ１３－ａｃｅｔａｔｅ，ＴＰＡ）作用于ＮＩＨ３Ｔ３细胞２４ｈ，流式细胞仪检测到细胞表面整合蛋白α５亚基含量增加５２．３％．Ｎｏｒｔｈｅｒｎ杂交方法测定结果亦表明整合蛋白α５亚基ｍＲＮＡ量增加，于２ｈ时达到高峰，为对照的４．１４倍．蛋白激酶Ｃ（ｐｒｏｔｅｉｎｋｉｎａｓｅＣ，ＰＫＣ）的活性增加趋势与之基本一致．运用ＰＫＣ的抑制剂鞘氨醇（ｓｐｈｉｎｇｏ－ｓｉｎｅ）和酷氨酸激酶（ｔｙｒｏｓｉｎｅｋｉｎａｓｅ，ＴＫ）抑制剂４，５，７－三羟基异黄酮（ｇｅｎｅｓｔｅｉｎ）进一步研究，发现两者均可抑制佛波酯对整合蛋白α５亚基表达的上调作用．提示佛波酯对ＮＩＨ３Ｔ３细胞整合蛋白α５亚基表达的调控与ＰＫＣ和ＴＫ均有关．
TPA is known to be a tumor promoter and able to activate PKC straightly, and integrin is a kind of cell adhesion molecules closely related to tumor metastasis. so the effect of TPA on the expression of integrin α 5 subunit in NIH3T3 cells was studied. Being treated by 100 nmol/L TPA for 24 h 52.3% increased expression of integrin α 5 subunit was detected by flow cytometer. Northern blot also showed that TPA induced the increase of integrin α 5 subunit mRNA. The maximal effect appeared at the end of 2 h and was 4.14 times of the control. At the smae time, the increase of PKC activity was consistent with the change of integrin α 5. And this effect of TPA could be inhibited by PKC inhibitor——genestein or TK inhibitor——sphingosine. These results implicated that the signal transduction pathway of induction of integrin α 5 subunit expression by TPA is related to PKC and TK.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1998年第6期780-783,共4页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金
上海市科委基金
上海市教委重点学科项目基金
