摘要
为探讨白细胞介素１对肾小球系膜细胞作用的细胞内信号转导机制，采用３Ｈ胸腺嘧啶参入、免疫沉淀及激酶磷酸化反应等方法，检测了白细胞介素１对分裂原活化蛋白激酶不同亚类的作用，并与血小板衍生生长因子的作用进行比较。结果发现，与血小板衍生生长因子的作用相反，白细胞介素１可在短时间内激活ｃｊｕｎ氨基末端激酶，但对ｐ４２／４４分裂原活化蛋白激酶无刺激作用，表明白细胞介素１在肾小球系膜细胞的作用可通过ｃｊｕｎ氨基末端激酶介导。
おhe signalling pathways by which IL1 exerts its effects in renal mesangial of subfamily of mitogenactivited protein kinases(MAPKs) stimulated by IL1.DNA synthesis in MsC was detected by 3HThymedine uptake.p42/44 MAPK activity was measured by phosphorylation of myelin basic protein.Cjun aminoterminal kinase(JNK)activity was measured by phosphorylation of GSTcjun immobilized to glutathioneagarose.The results revealed that IL1β seems to be a very weak stimulatory cytokine for DNA synthesis in MsC.IL1β(10 ng/ml)stimulated JNK activity by 2～3 fold in 15 min and return to normal by 1～2 hour.However,IL1β had no affect on p42/44 MAPK activity. In contrast to IL1β,polypeptide pletletderived growth factor(PDGF)rapidly activated p42/44 MAPK,but no effect on JNK from 15 min to 2 hours. These results suggested that effects of IL1 in MsC may be mediated through activation of JNK.The different signal pathway may play the distinct role on MsC proliferation and differentiation.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第4期300-303,共4页
Chinese Journal of Immunology
基金
国家自然科学基金
