摘要
目的观察白细胞介素 - 10 (IL - 10 )对 IL - 1β诱导的人系膜细胞 (HMC)前列腺素 E2 (PGE2 )释放及环氧化酶 - 2(COX- 2 )基因和蛋白表达的影响。方法应用放射免疫测定法检测 HMC培养上清中 PGE2 ,应用 RT- PCR和 western blot检测 COX- 2 m RNA和蛋白水平。结果 1IL - 1β显著上调 PGE2 释放及 COX- 2基因和蛋白的表达 (P均 <0 .0 1) ;2 IL - 10对基础状态下 PGE2 释放及 COX- 2基因和蛋白表达无明显影响 (P>0 .0 5 ) ;3 IL - 10可呈剂量依赖性地下调 IL - 1β诱导的 PGE2 释放及 COX- 2 m RNA和蛋白表达 (P<0 .0 1)。结论 IL - 10抑制 IL - 1β诱导的 HMC PGE2 释放及 COX- 2表达 ,提示 :IL -
Objective To investigate the effect of IL 10 on IL 1β induced prostaglandin E 2 (PGE 2) release and cyclo oxygenase 2 (COX 2) expression in human mesangial cells and to examine whether IL 10 has effect on the biological function of IL 1β Methods The PGE 2 concentration in supernatants of HMC was measured by radio immunoassay The COX 2 mRNA expression and protein production were measured by RT PCR and western blot, respectively Results HMC produced increased amounts of PGE 2 and COX 2 after treatment with IL 1β IL 10 inhibited IL 1β elicited PGE 2 production, as well as COX 2 mRNA and protein expression in a concentration dependent fashion Conclusions The results indicated that IL 10 reversed the IL 1β induced release of PGE 2 and expression of COX 2 These data suggest that IL 10 could exert anti inflammatory actions at several levels, which not only inhibits the production of pro inflammatory cytokines but also suppresses their biological function
出处
《免疫学杂志》
CAS
CSCD
北大核心
2001年第6期421-424,共4页
Immunological Journal
基金
江苏省卫生厅科研基金 (H970 9)
南京医科大学校基金 (NY990 2 8)资助项目
