摘要
为了探讨ｒｈＴＮＦα对人胚胎胰岛分泌ＩＬ６以及对胰岛分泌功能的影响，观察了ｒｈＴＮＦα对原代培养的人胚胎胰岛细胞的作用特点。结果表明：①经ｒｈＴＮＦα刺激后胰岛培养上清ＩＬ６含量显著升高，在一定范围内呈剂量依赖关系；②斑点杂交结果显示ｒｈＴＮＦα刺激的胰岛ＲＮＡ中ＩＬ６ｍＲＮＡ含量高于对照组；③人胚胎胰岛受ｒｈＴＮＦα刺激后培养上清胰岛素含量降低；④人胚胎胰岛受ｒｈＴＮＦα刺激后培养上清胰高血糖素分泌能力明显升高，分泌趋势与ＩＬ６相似。
おL6 production in vitro.The islets from fetal pancrease was separated by collagenase type V(0.5 mg/ml) and cultured in vitro.The islets were exposed for 48 h to culture medium alone or with different concentration of rhTNFα.The supernatants of culture of human fetal islets were assayed for IL6,insulin and glucangon.The results showed ①IL6 activity was increased 14.5 folds(78～1 131 mU/islet) when islets were exposed to rhTNFα(200 U/ml);②IL6 mRNA in human fetal islet exposed to rhTNFα is higher than control in dot hybridization;③Soluble insulin released to supernatants was slightly decreased(0.83～0.62 IU/islet) and cellular insulin within islet slightly increased(0.86～0.96 IU/islet);④Glucagon secretion was significantly increased 3.2 folds(1.02～3.46 pg/islet).The results suggest that pancreatic islets produce IL6 is upregulated by rhTNFα.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第4期276-278,共3页
Chinese Journal of Immunology
基金
国家自然科学基金
