摘要
目的探讨脑肿瘤细胞增殖活性及凋亡与人类白细胞抗原－Dr（HLA－Dr）表达的相互关系。方法用免疫组化和原位凋亡细胞检测方法现察分析了44例原发性脑肿瘤。结果增殖细胞核抗原（PCNA）、Ki－67抗原（Ki－Dr）阳性细胞和凋亡细胞阳性率均为100％，29例（67．2％）表达HLA－Dr。PCNA和Ki－67阳性细胞密度及HLA－Dr阳性率和阳性细胞含量均随肿瘤恶性程度增高而升高，凋亡细胞密度随肿瘤恶性程度增高而减少。HLA－Dr表达水平与PCNA和Ki－67阳性细胞密度呈平行升高，HLA－Dr－、＋、＋＋、＋＋＋组间的凋亡细胞密度无显著性差异。结论本组结果提示以上四种指标均能较客观反映脑肿瘤的恶性程度，HLA－Dr表达水平与脑肿瘤细胞增殖速度密切相关，细胞增殖与凋亡失衡可能是脑肿瘤发生发展的重要环节。
To study the relationship among Proliferative activity. apoptosis and HLA-Dr expressive level in the cells of human brain tumors. Methods Forty-four primary brain tumors were analyzed by in situs marking apoptosis and immunohistochemistry. Results All of the tumors were positive for PCNA. Ki-67 and apoptosis(100%). 29(67. 2%) expressed HLA-Dr. Either the densities of cells positive for PCNA and Ki-67 or the positive rate and positive cell number of HLA-Dr rose along with the increase in tumor malignancy. The density of apoptotic cells reduced along with the increase in the malignancy. The level of HLA -Dr expression rose parallelly along with the increase in densities of cells positive for PCNA and Ki-67. There was not significant difference among densities of apoptotic cells of HLA -Dr-, +, groups. Conclusions The above results suggest that all of the four targets can objectively reflect malignant grade of brain tumors. The level of HLA-Dr expression correlates closely with proliferative speed of brain tumor cells. The imbalance between cell proliferation and apoptosis may be a key link of formation and progression of brain tumors.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
1998年第3期129-132,共4页
Chinese Journal of Nervous and Mental Diseases
基金
天津市教委重点学科基金
