摘要
目的观察阿米替林对分支选择结扎模型（spared nerve injury，SNI）大鼠脊髓星形胶质细胞激活程度的影响。方法120只雄性SD大鼠，分为对照组（A）、SNI模型组（B）、单纯给药组（C）、SNI模型＋药物干预组（D），经腹腔分别给予0．2ml生理盐水（A、B）、10mg／kg阿米替林（C、D），每日2次。术后1、3、5d取L3～L6脊髓，分别进行星形胶质细胞激活标志物GFAP免疫荧光染色、Western blot和半定量RT—PCR检测，同时观察机械痛阈值改变。结果与A组相比，B组大鼠机械痛阈值随时间下降明显（P〈0．05），GFAP蛋白表达（0．38±0．24，0．84±0．31，1．36±0．43）和mRNA表达（0．55±0．14，0．86±0．16，1．32±0．32）随时间逐渐增强；C组各项指标与A组比较无明显差异；D组大鼠给药后3、5d机械痛阈值停止下降[（4．5±1．5）、（4．9±1．4）g]，GFAP蛋白（0．79±0．25，0．77±0．23）和mRNA（0．63±0．06，0．64±0．17）表达较A组略增高，但不及B组（P〈0．05）。结论阿米替林可能通过抑制星形胶质细胞激活，发挥治疗神经病理性疼痛作用。
Objective To investigate influence of amitriptyline on astrocytic activation in spinal cord of rats after spared nerve injury （SNI）. Methods A total of 120 male SD rats were randomly divided into 4 groups with 30 rats in each group ： control （ A）, SNI （ B）, amitriptyline （ C）, and SNI ＋ amitriptyline （D） groups, which respectively were treated with intraperitoneal injections of 0. 2 ml normal saline （A and B） , 10 mg/kg amitriptyline （ C and D）, bid. The L3 to L6 segment of the spinal cord was isolated respectively at 1, 3 and 5 d after surgery. Expression of glial fibrillary acidic protein （GFAP）, marker of astrocytes, was determined by immunofluorescence, Western blot assay and semiquantitative RT-PCR. The changes of mechanical pain threshold were measured. Results Compared with control, group B had a markedly decreased rat mechanical pain threshold （ P 〈 0. 05） and an obviously high expression of GFAP at both protein （0.38 ±0.24, 0.84 ±0.31 and 1.36 ±0.43） and mRNA （0.55 ±0.14, 0. 86±0.16, 1.32 ±0.32） levels at the corresponding time points. However, there was no significant change on these indexes between groups A and C. Mechanical pain threshold of rats in group D did not change any more in 3 and 5 d after surgery （4.5 ± 1.5 and 4.9 ± 1.4 g）. In addition, the expression of GFAP at both protein （0.79 ± 0.25, O. 77± 0.23） and mRNA （0.63 ±0. 06, 0. 64 ±0.17） levels in group D were higher than those in control but still lower than those of group B （P 〈 0. 05 ） in 3 and 5 d after surgery. Conclusion Amitriptyline inhibits the activation of astrocytes in SNI animals, which maybe one of its mechanisms in the treatment of neuropathic pain.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2009年第7期611-614,共4页
Journal of Third Military Medical University
关键词
神经性疼痛
星形胶质细胞
抗抑郁药
GFAP
neuropathic pain
astrocyte
antidepressant
glial fibrillary acidic protein
