摘要
目的应用膜片钳全细胞记录技术,探讨觉醒肽orexinA对小鼠前额叶皮层PL区细胞的作用。方法制备小鼠前额叶皮质脑片,在膜片钳全细胞记录模式下,观察记录PL区锥体神经元和中间神经元的电生理学特点及其对orex-inA的反应情况。结果54例锥体神经元对orexinA总反应率为51.9%。36例Ih(+)锥体神经元对orexinA反应率为66.7%,18例Ih(-)锥体神经元对orexinA反应率为22.2%,两者差异具有统计学意义(P<0.01);15例中间神经元对orexinA均无反应。结论orexinA对前额叶皮层PL区锥体神经元具有一定兴奋性效应,并且表现该兴奋性反应的细胞倾向分布于具有Ih电流的锥体神经元中。
Objective To investigate the effect of exogenous orexinA on the pyramid neurons and interneurons in the prefrontal cortex prelimbic （PL） area. Methods The pyramid neurons and interneurons in PL area of Kunming mice were selected from prefrontal cortex slices by infrared visual patch clamp technique. The morphology and electrophysiological features of the pyramid neurons and interneurons were observed. The effect of exogenous orexinA at a concentration of 400 nmol/L on these cells was studied using the whole cell configuration. Results The pyramid neurons were large, pyramidal in cell body with clear apical dendrites extending vertically and several basal dendrites radiating. The interneurons were comparatively smaller and had several processes from cell body. In current clamp mode, all 54 pyramid neurons having been recorded showed frequency adaption, and the 15 recorded interneurons discharged rapidly and had no frequency adaption. While in voltage clamp mode, 36 pyramid neurons were regarded as Ih （ ＋ ） pyramid neurons for recorded hyperpolariztionactivated cation current, and the left 18 and 15 interneurons were Ih （ - ）. Exogenous orexinA had a total reaction rate of 51.9% on 54 recorded pyramid neurons under current clamp, and a rate of 66.7% on 36 Ih （ ＋ ） pyramid neurons and of 22.2% on the Ih（ - ） pyramid neurons under voltage clamp. All 15 recorded interneurons had no reaction to exogenous orexinA under either mode. Conclusion OrexinA plays excitatory effect on pyramid neurons in the prefrontal cortex PL area, and this effect is much more noticeable in pyramid neurons with Ih currents.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2009年第7期561-564,共4页
Journal of Third Military Medical University
基金
国家自然科学基金(30670668)
全军医学科研"十一五"计划面上项目(06MA195)~~
