摘要
目的:探讨癫痫对大鼠海马线粒体细胞色素氧化酶(COX)的mtDNA和nDNA编码亚基Ⅲ、Ⅳ表达的影响。方法:将成年雄性Wistar大鼠40只随机分为生理盐水对照组,癫痫持续状态(SE)后急性期(3 h)、静止期(7 d)、慢性期(45 d)组和注射匹鲁卡品(PILO)但未出现SE组,每组8只。荧光实时定量PCR和West-ern blot分别检测海马线粒体COXⅢ、ⅣmRNA和蛋白的表达。结果:COXⅢmRNA和蛋白在SE后3 h表达明显升高(P<0.001,P<0.01),7 d时降到对照水平,45 d显著降低(P<0.001,P<0.01);COXⅣmRNA和蛋白在3 h时表达较对照组升高,但差异无统计学意义(P>0.05),7 d和45 d时下降至对照水平。注射PILO但无SE组与对照组结果类似。结论:颞叶癫痫海马cox功能紊乱与痫性发作相关,线粒体编码的基因更易受痫性发作的影响。
Objective： To investigate the effect of epilepsy on expressions of cytochrome oxidase（COX） subnuits m 〈cox Ⅲ ） and Ⅳ （COX Ⅳ ） encoded by mtDNA and nDNA in the rat hippocampns. Methods： Forty male Wistar rots were randomly divided into the saline control group, the acute period group （3 h）, the silent period group （7 d）, the chronic period group（45 d） after status epilepticns（SE） and the group which received pilocarpine （PILO） but did not develop SE（ n = 8 per group）. Expressions of COX m and COX IV mRNA and protein in the rat hippocampns were respectively determined by real time quantitative PCR（RQ-PCR） and Western blot. Results： COX m mRNA and protein were significantly increased in the group at 3 h after SE（ P 〈 0.001, P 〈0.01 ） compared with the control group. The silent group presented a similar level of expression of COX m, but the chronic group showed a significantly decreased level in respect to the control group （ P 〈 0. 001, P 〈 0.01 ）. A slightly increased but not significant expression of COX1V was found in the acute period group（ P 〉 0.05）, then it slightly decreased and remained constant during the following days of epilepsy compared with the saline-treated animals. Animals that did not respond to PIIX） in our study showed similar results with those of the control group. Conclusion： Dysfunction of COX in the hippocampus is related to prolonged seizure during experimental temporal lobe epilepsy and the mitochondria are vulnerable to epilepsy.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第8期817-820,824,共5页
Journal of Shandong University:Health Sciences
