摘要
目的克隆红曲霉和土曲霉洛伐他汀合成酶调控基因lovE和mkH,并进行序列分析和同源性比较。方法根据GeneBank土曲霉和红曲霉洛伐他汀合成酶基因序列设计引物,以土曲霉和红曲霉基因组DNA为模板PCR扩增lovE和mkH基因并克隆到pMD19Tsimple载体。利用DNAMAN等软件以及互联网资源对lovE和mkH测序结果与其编码的氨基酸序列进行分析比对。结果分别扩增得到1 512 bp和1 464 bp的目的片段lovE和mkH。结论lovE和mkH同源性很高,并与GeneBank中相关已知序列基本相同,是洛伐他汀生物合成酶调控基因,且其表达产物为GAL4类转录因子。
Objective To clone Aspergillus terrus and Mortascus lovastatin biosynthesis regulatory genes lorE and mkH, analyze and align the sequences. Methods According to well-known lovastatin synthase gene sequences from GenBank, primers were designed to amplify from genomic DNA and clone lovastatin biosynthesis regulatory genes lorE and mkH into vector pMD19T simple. Alignment and analysis of sequencing results lovE, rnkH and their encoding animo acids sequences were performed through internet resources and some softwares like DNAMAN. Results Target fragments lorE and mkH, 1 512 bp and 1 464 bp in length respectively, were amplified successfully. Conclusion Highly homologous lovE and mkH, almost the same as related well-known sequences in GenBank, are regulatory genes and their encoding proteins are GAL4-like transcriptional factors.
出处
《广东药学院学报》
CAS
2009年第1期83-87,共5页
Academic Journal of Guangdong College of Pharmacy
关键词
土曲霉
红曲霉
洛伐他汀
转录因子
调控基因
Aspergillus terrtts
Monascus
lovastatin
transcriptional factor
regulatory gene
