摘要
目的观察南瓜蛋白(CUS)对人胰腺癌细胞系PANC-1的细胞毒性和化疗增敏作用。方法采用四甲基偶氮唑蓝(MTT)比色法,观察不同浓度(量效)的CUS及作用不同时间(时效)对胰腺癌细胞PANC-1的细胞毒性;采用Chou Talalay联合指数法判断CUS增加胰腺癌细胞对吉西他滨(GEM)的化疗敏感性。结果CUS对胰腺癌PANC-1细胞具有显著的增殖抑制作用,0.3125,0.625,1.25,2.5,5.0,10.0,20.0,40.0,80.0μg/mL的CUS作用于胰腺癌细胞24 h,其抑制率分别为9.16%,11.9%,14.4%,20.2%,32.0%,36.4%,44.1%,50.3%和58.6%,呈现明显的剂量依赖性;随着作用时间的延长,其抑制率明显增加,24,48,72 h的半数抑制浓度(IC50)分别为40.24,8.24,1.17μg/mL,呈现明显的时间依赖性。两药在不同效应时各自所需浓度之间相互作用均为协同作用(CI<1),单吉西他滨用于胰腺癌细胞的IC50为36.76 nmol/L,CUS与其合用时的IC50为12.14 nmol/L,合用比单用时吉西他滨的剂量减少67.0%。结论CUS对胰腺癌的细胞毒性作用呈明显的剂量依赖性和时间依赖性,与吉西他滨联合使用可增加其细胞毒作用,起到化疗增敏效应。
Objective To investigate the cytotoxic and chemo-sensitivity enhamcement effect of cucurmosin （CUS） on pancreatic cancer cell line PANC-1 in vitro. Methods The cytotoxic effect of the different concentrations of CUS on PANC-1 cells in different time periods （24, 48 and 72 hours ） was detected by means of MTT assay, and the ehemo-sensitivity enhancement effect of CUS on gemcitabine （ GEM ） was evaluated by combination-index method of Chou and Talalay. Results Cucnrmosin significantly inhibited the proliferation of pancreatic cancer cell line PANC-1 in dosage-and time-dependent manner. After exposure to 0. 3125,0. 625,1.25,2.5,5.0,10.0,20.0,40.0 and 80μg/mL of CUS, the inhibitory rate of the cell growth at 24 hours was 9.16% , 11. 9% , 14.4% ,20.2% ,32.0% ,36.4% ,44.1% ,50.3% and 58.6% respectively. The 50% inhibitory concentration （ IC50 ） at 24,48 and 72 h was 40.24μg/mL, 8.24μg/mL and 1. 17 μg/mL respectively. The combination of CUS and GEM at different concentrations showed synergie effect on inhibiting PNCA-1 cell growth （ CI 〈 1 ） . The 50 % inhibitory concentration （ IC50 ） of GEM decreased from 36.76 nmol/L to 12. 14 nmol/L（ less than 67.0% ） when combined with CUS. Conclusions Cucurmosin can significantly inhibit the proliferation of PANC-1 cells in dosage-and time-dependent manner in vitro, and combination with gemcitabine increases its cytotoxic action and provides chemo-sensitivity enhancement effect on pancreatic cancer cell line PANC-1.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2009年第3期233-236,共4页
China Journal of General Surgery
基金
国家自然科学基金资助项目(30772587)
福建省自然科学基金资助项目(C0510012)
