摘要
采用微生物酶转化法制备L-半胱氨酸具有周期短、成本低、区域和立体选择性强、反应条件容易控制、环境友好等特点,与传统的毛发水解以及化学合成工艺相比显示出明显的优越性。本文从假单胞菌产酶条件和酶学性质、DL-ATC生物转化途径、固定化细胞转化工艺、基因工程菌的研究、以及L-半胱氨酸脱巯基酶的研究等5个方面介绍了国内外关于生物转化DL-2-氨基-Δ2-噻唑啉-4-羧酸(DL-ATC)合成L-半胱氨酸的研究进展。
The microbial transformation method for L- cysteine production shows evident advantages, because of its short cycle time, low cost, high regio and stereoselectivity, easy control of reaction condition, and environment - friendly. Recently, studies on the bioeonversion of DL - 2 - amino - △^2- thiazoline - 4 - carboxylie acid （ DL - ATC） to L - cysteine by intracellular enzymes were reported. The research progresses on L - cysteine production by microbial bioeonversion, especially pseudomonas sp. , or its crude enzyme extract are summarized. The applications of immobilization technology in the biotransformation of DL -ATC to L -cysteine are introduced. The genetically engineered bacteria and the study progresses of L - cysteine desulfhydrase were also discussed.
出处
《氨基酸和生物资源》
CAS
2009年第1期58-63,共6页
Amino Acids & Biotic Resources
基金
浙江省科技厅重大项目资助(No.2006C13001)
浙江省重中之重学科开放基金资助
