摘要
建立了假单胞菌 TS1138菌株L-半胱氨酸酶法合成途径的动力学模型。在L-半胱氨酸合成途径中有L-半胱氨酸合成酶和L- 半胱氨酸脱巯基酶两种酶参与作用,它们的动力学模型是基于米氏动力学建立的,L- 半胱氨酸对L- 半胱氨酸合成酶有非竞争性抑制作用。通过测定L- 半胱氨酸酶法合成过程中的中间产物和终产物的浓度,利用动力学模型进行了L- 半胱氨酸合成途径的代谢控制分析。弹性系数首先可以从动力学方程中求出,根据代谢控制分析理论,流量控制系数由弹性系数求出。研究发现,在 L- 半胱氨酸酶法合成过程中,L- 半胱氨酸合成酶和L- 半胱氨酸脱巯基酶的流量控制系数存在一个交叉点。
To optimize the flux through a metabolic pathway, in order that the rate of production of a specific cellular metabolite is maximized, it is important to understand how the metabolic flux is controlled by the individual reactions. The rates of these individual steps are in turn dependent on the conditions of the cultivation, metabolic effectors, and on the environment. Metabolic engineering has been defined asthe purposeful modification of intermediary metabolism by recombinant DNA techniques. It offers the possibility to design in a systematic fashion new and better strains with improved productivity of both traditional metabolites and novel compounds. It is a multidisciplinary field applying information and techniques from biochemistry, genetics, molecular biology, cell physiology and chemical engineering.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第1期219-224,共6页
Food Science
基金
天津市科委应用基础研究重点基金项目(05YFJZJC00901)
