摘要
目的:探讨活性炭纳米粒子(activated carbon nanoparticles,ACNP)对人胃癌BGC-823细胞的作用。方法:将ACNP以不同浓度和时间作用于BGC-823细胞,通过MTT试验观察ACNP对细胞增殖的抑制作用;测定培养上清液乳酸脱氢酶(LDH)活性,判断细胞损伤状况;用甲基绿-派洛宁染色法及透射电镜观察细胞凋亡形态;用流式细胞术检测细胞凋亡率和细胞周期。结果:ACNP能明显抑制BGC-823细胞的增殖,呈剂量和时间依赖性,作用24,48,72h后的半数抑制浓度(IC50)分别是1.57,1.18,0.87g/L。低浓度ACNP(0.1,0.2g/L)作用24h后,LDH漏出量与对照组相比没有显著差异。0.1g/L的ACNP作用24h后,细胞出现凋亡特征的形态学改变。0.1,0.2g/L的ACNP作用24h后,细胞凋亡率分别为(5.01±1.16)%、(8.21±1.63)%,与对照组(2.48±0.58)%比较均有显著性差异;ACNP作用组的S期细胞所占比例明显增高。结论:ACNP抑制BGC-823细胞的增殖,可使细胞阻滞于S期,诱导细胞凋亡。
Objective ：To investigate the effect of the activated carbon nanoparticles（ACNP） on the human gastric carcinoma cell line BGC-823 in vitro. Methods： BGC-823 cells were treated with ACNP at various concentrations for different durations. The inhibitory effect of ACNP on the proliferation of BGC-823 cells was observed by MTr assay. The lactate dehydrogenase（LDH） leakages in the culture medium were determined with LDH activity detection kit. The apoptosis-related alterations in morphology were observed by methyl green-pyronin staining and transmission electron microscopy（TEM）. The apoptotic rate and the cell cycle of BGC-823 cells were examined by flow cytometry（FCM）. Results： ACNP significantly inhibited the proliferation of BGC-823 cells in a dose- and time-dependent manner. The 50% inhibitory concentrations （ICso） after 24, 48 and 72 hours were 1. 571 1.18 and 0. 87 g/L,respectively. The LDH leakages at concentrations of 0.1 g/L and 0.2 g/L were not higher than that of the control group at 24 hours. The cells treated with 0.1 g/L ACNP for 24 hours exhibited characteristics of apoptosis. The apoptotic rates at concentrations of O. 1 g/L and 0.2 g/L were（5. 01±1.16） % and （8.21 ± 1.63 ） %, respectively, both of which were significantly higher than that of the control group （2.48±0.58 ） % at 24 hours. In ACNP group, the percentage of cells at S phase was higher than that in control group（ P 〈 0.01 ）.Conclusion： The ACNP could inhibit proliferation, cause S-phase arrest and induce apoptosis of BGC-823 cells in vitro.
出处
《军事医学科学院院刊》
CSCD
北大核心
2009年第1期29-32,80,共5页
Bulletin of the Academy of Military Medical Sciences
基金
国家"973"项目(2006CB705602-7)
国家"863"项目(2006AA03Z333)
关键词
活性炭纳米粒子
人胃癌BGC-823细胞
增殖
凋亡
activated carbon nanoparticles
human gastric carcinoma cell line BGC-823
proliferation
apoptosis
