摘要
目的探讨Ｋｕｐｆｅｒ细胞对大鼠实验性肝癌形成的影响。方法应用免疫组化和原位分子杂交技术，对单纯用化学致癌剂二乙基亚硝胺（ＤＥＮＡ）及氯化钆（ｇａｄｏｌｉｎｉｕｍｃｈｌｏｒｉｄｅ，ＧＣ）填充Ｋｕｐｆｅｒ细胞后，同时给以ＤＥＮＡ所引起的大鼠肝癌形成过程中的增殖细胞核抗原（ＰＣＮＡ）、表皮生长因子（ＥＧＦ）、ｒａｓｐ２１、ｐ５３蛋白及ｃｍｙｃ基因表达进行了对比研究。结果结果显示：ＰＣＮＡ、ＥＧＦ表达在两组动物肝组织中未见明显差异；ＧＣ＋ＤＥＮＡ组ｒａｓｐ２１表达阳性率明显高于ＤＥＮＡ组；ｐ５３蛋白的表达在ＧＣ＋ＤＥＮＡ组略高于ＤＥＮＡ组，但出现时间则远较ＤＥＮＡ组为早，前者出现于第１１周，后者则在第１９周；ｃｍｙｃ基因表达强度ＧＣ＋ＤＥＮＡ组明显高于ＤＥＮＡ组。结论上述结果提示Ｋｕｐｆｅｒ细胞能够降低化学致癌剂对肝细胞的毒性损伤，从而在一定程度上对肝细胞的癌变过程可能具有抑制作用。
Objective In order to explore the influence of Kupffer cells on experimental hepatocarcinogenesis. Medoths A comparative study on the expression of proliferating cell nelclear antigen (PCNA), epidermal growth factor (EGF), ras P21, p53 protein and c myc gene by means of immunohistochemistry or in situ hybridization during the diethylnitrosamine (DENA) induced hepatocarcinogenesis performed in rats with and without pretreatment with gadolinium chloride (GC) which might effectively block the activity of Kupffer cells. Results There was no marked difference in the expression of PCNA and EGF in the liver tissue between the above two groups animals. The positive rate of ras P21 was markedly higher in the GC+DENA group than in the DENA group. The positive rate of p53 protein in GC+DENA group was slightly higher than that of the DENA group, however, the appearance of expression was markedly earlier in the former group (in the 11th and the 19th week respectively). The expression intensity of c myc gene was markedly higher in GC+DENA group that in DENA group. Conclusions The results suggest that Kupffer cells can reduce hepatotoxicity induced by chemical carcinogen, and Kupffer cells may play an inhibitory effect on the process of hepatocarcinogenesis.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1998年第2期102-104,共3页
Chinese Journal of Pathology
关键词
肝肿瘤
KUPFFER细胞
肿瘤形成
Liver neoplasms, experimental Diethylnitrosamine Gadolinium Proto oncogene protein P21(ras) Protein p53 Kupffer′s cell
