摘要
目的探讨巨噬细胞(Mφ)、肝脏自然杀伤(NK)细胞在原发性肝细胞癌(HCC)及肝硬化组织中的数量和分布规律,及其两者的相互关系。方法应用免疫组织化学二步法检测60例HCC,62例单纯性肝硬化,23例正常肝组织中的巨噬细胞标志物CD68和NK细胞标志物CD57的表达,对阳性细胞数进行形态定量比较与相关分析。结果(1)各组Mφ平均数从高到低为:癌旁组织、肝硬化、正常肝、HCC(P<0.05);NK细胞平均数从高到低为:HCC、癌旁组织、正常肝、肝硬化(P<0.05)。(2)HCC中Mφ细胞数量随分化程度降低而减少(P<0.05);NK细胞数量与组织学分级无明显关系。(3)HCC和癌旁组织中Mφ细胞分布与临床TNM分期无关;HCC中NK细胞随着临床分期的发展有下降的趋势(P<0.05)。(4)HCC中15月内有转移组的Mφ细胞数少于无转移组(P<0.05);15月内转移复发组HCC和癌旁的NK细胞计数均明显少于无转移复发组(P<0.01)。(5)在HCC中Mφ、NK细胞数呈线性正相关(r=0.344,P<0.05)。结论Mφ数量与HCC分化程度密切相关,并与肝脏NK细胞数量呈正相关。Mφ和NK细胞计数可能是反映机体抗肿瘤免疫状态和生物学行为以及判断患者预后的重要指标。
Objective To investigate the number and distribution of macrophages(M_φ) and natural killer(NK) cells in hepatocellular carcinoma(HCC), paraneoplastic,cirrhosis and normal liver tissues. Methods Surgical specimens from 60 cases of HCC, 62 cases of cirrhosis and 23 cases of normal liver tissues were investigated by immunohistochemical staining of CD_(68) and CD_(57) with polymer detection system. Results (1)The order of the number of Mφ from the highest to the lowest was: paraneoplastic, cirrhosis, normal liver, HCC(P<0.05); and NK cells: HCC ,paraneoplastic , normal liver ,cirrhosis(P<0.05). (2)The number of M_φ decreased successively with the decrease of the HCC differentiation(P<0.05); There was no relationship between the number of NK cells in HCC and histological grade. (3)There was no relationship between the number of M_φ in HCC and clinical TNM stage ; The number of NK cells in HCC had degressive current with the clinical TNM stage (P<0.05). (4) The number of M_φ in the cases with metastasis in 15 months was significantly lower than that without metastasis(P<0.05);The number of NK cells in HCC and its paraneoplastic tissues in the group with metastasis in 15 months were both significantly lower than that without metastasis(P<0.01). (5)There was a linearity positive correlation between the number of M_φ and NK cells in HCC(r=0.344,P <0.05). Conclusion The number of M_φ has a closed correlation with the HCC differentiation, and positive correlation with the number of NK cells. The number of M_φ and NK cells might be important markers to estimate the immune status and useful factors to predict the prognosis of HCC patients.
出处
《现代肿瘤医学》
CAS
2005年第3期307-310,共4页
Journal of Modern Oncology
基金
广西科学基金资助项目(桂科回:0342021)
