摘要
目的探讨红细胞膜脂肪酸成分和膜流动性与糖尿病视网膜病变（ＤＲ）之间的关系。方法采用高效液相色谱法和荧光偏振技术测定２９例正常人和６３例非胰岛素依赖型糖尿病（ＮＩＤＤＭ）患者红细胞膜脂肪酸成分和膜微粘度。结果ＮＩＤＤＭ病人红细胞膜花生四烯酸（Ｃ２０∶４ｎ－６）含量及百分组成明显低于正常对照组，而膜微粘度则明显高于对照组，且在伴有ＤＲ组其膜微粘度又明显高于不伴有ＤＲ组。患者膜微粘度与膜软脂酸（Ｃ１６∶０）和硬脂酸（Ｃ１８∶０）百分组成呈明显正相关，而与花生四烯酸和廿二碳六烯酸（Ｃ２２∶６ｎ－３）呈明显负相关。结论ＮＩＤＤＭ病人红细胞膜脂酸组成对其膜流动性降低有重要影响，而后者对ＮＩＤＤＭ微血管病变的发生和发展可能起重要作用。
Objective To investigate the relationship between erythrocyte membrane fluidity and fatty acid components and diabetic retinopathy (DR). Methods We examined the fatty acid components and fluidity of erythrocyte membrane in 29 normal subjects and 63 patients with type Ⅱ diabetcs mellitus (NIDDM) with and without DR by a high performance liquid chromatographic (HPLC) method and fluorescence polarization technology. Results The contents and constituting percentage of erythrocyte membrane arachidonate (C 20∶4 n-6 ) were significantly lower in the diabetics than the controls. The diabetics had significantly increased erythrocyte membrane microviscosity as compared with the controls, and the membrane microviscosity was higher in diabetics with DR than those without DR. The microviscosity values were positively correlated with palmitate (C 16∶0 ) and stearate (C 18∶0 ) levels of erythrocyte membrane and negatively correlated with the arachidonate (C 20∶4 n-6 ) and docosahexaenoate (C 22∶6 n-3 ) in NIDDM patients. Conclusion Erythrocyte membrane fatty acid component may contribute to the alteration of membrane fluidity in NIDDM patients and the alteration of membrane fluidity may play an important role in the development of diabetic microangiopathy.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1998年第2期88-90,共3页
Chinese Journal of Internal Medicine
基金
国家教委博士生经费
重庆市中青年科技专家基金
关键词
红细胞膜
脂肪酸类
膜流动性
糖尿病视网膜病
Erythrocyte membrane Fatty acids Membrane fluidity Diabetic retinopathy
