摘要
应用ＰＣＲ技术优化人单核细胞趋化蛋白－１（ＭＣＰ－１）基因的５端翻译起始区，并将其亚克隆入大肠杆菌表达载体ｐＢＶ２２０中，经ＤＮＡ测序证实优化成功，且在大肠杆菌中表达出ＭＣＰ－１蛋白，表达产物约占菌体总蛋白的１０％左右，Ｗｅｓｔｅｒｎｂｌｏｔ检测表明，表达产物可与ＭＣＰ－１抗体发生特异性反应。
he 5translation initiation region of human monocyte chemoattractant protein1(MCP1) was optimized by PCR and the optimized MCP1 gene was cloned into pBV220. DNA sequence indicated that it was correct. MCP1 was expressed successfully at the level of about 10% of total proteins in E.coli. Western blot analysis showed that the expressed product was reacted specifically with antiMCP1 antibodies.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第1期18-21,共4页
Chinese Journal of Immunology
关键词
人单核细胞趋化蛋白-1
PCR
原核表达
Human monocyte chemoattractant protein1 PCR Gene cloning and expression
