摘要
人单核细胞趋化蛋白－１（ＭＣＰ－１）是由多种细胞产生的一种趋化因子。它不但能趋化单核细胞还能激活单核细胞，在机体防御、炎症恢复和抗肿瘤等方面起重要作用。本文利用ＲＴ－ＰＣＲ方法克隆了人ＭＣＰ－１ｃＤＮＡ，经核苷酸序列测定，除第１２位密码子为ＴＧＴ与国外报道的ＴＧＣ不同、但编码相同的氨基酸半胱氨酸外，其余序列完全相同。
In order to clone the cDNA of the monocyte chemotactic protein-1(MCP-1 ),we have synthesized a pair of oligonucleotide acid to amplify the MCP-1 gene with RT-PCR. Total mRNA was isolated from PHA stimulated human PBMC,and followed by reverse transcription into cDNA. Then PCR was used to amplify the MCP-1 gene.The PCR product was cloned into pUC19 and sequenced. The results indicate that the cloned gene is almost the same as the published MCP-1 gene except for the 12 coden altered from TGT to TGC but still encoding the same amino acid cystein.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1996年第3期20-23,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家"863"青年基金
