摘要
本研究利用ＰＣＲ技术将人单核细胞趋化蛋白－１基因（ＭＣＰ－１）５′端翻译起始区进行优化改构。结果表明，改构后的ＭＣＰ－１基因克隆入大肠杆菌表达载体ｐＢＶ２２０中，ＤＮＡ测序证实正确。上述结果为进一步研究ＭＣＰ－１的高效表达奠定了基础。
The present study was designed to optimize the 5′ translation initiation region of human monocyte chemoattractant protein-1(MCP-1).The results showed that the optimized MCP-1 gene was cloned into pBV220,and DNA sequence indicated that it was correct.This lays the basis of further study on efficient expression of MCP-1.
出处
《白求恩医科大学学报》
CSCD
1997年第6期674-677,共4页
Journal of Norman Bethune University of Medical Science
