摘要
对杂交水稻威优35,威优64和常规稻78130强弱势粒ATP酶活性的测定、考种及稻穗不同部位枝梗ATP酶的电镜细胞化学观察表明,水稻灌浆期间籽粒ATP酶活性与灌浆速度呈正相关。ATP酶主要定位于稻穗枝梗筛管细胞质膜和细胞间隙周围的细胞壁表面,并具有较高的活性。灌浆前期,稻穗上部枝梗筛管细胞质膜上的ATP酶活性比下部强;其韧皮组织的胞间连丝比下部多;其韧皮部中可观察到大量物质从伴细胞向细胞间隙转移。在稻穗下部则几乎没有观察到这种现象。
The hybrid rice Weiyou 35, Weiyou 64 and cv. 78130 were served as experimental materials. Both vigorous and weak grains were used for testing the ATPase activity and for being weighed in the laboratory. The branches of various parts of the rice spike were taken to make the observation of cytochemistry under an electron microscope. The results show that there is a positive correlation between the ATPase activity of the grain and the filling velocity. The ATPase is chiefly localized at the plasmolemma of the sieve cells of the branches of rice spikes and on the surface of the cell wall surrounding the intercellular space, having higher activity. The ATPase activity at the plasmolemma of the sieve cells of the branches of the upper part is higher than that of the basal part. The plasmodesmata in phloem tissues of the branches of the upper part of the spike are more than those of the basal part. In the phloem of branches of the upper part of the spike a large amount of inclusion transferred from the companion cells to the intercelluar spaces, but this phenomenon is hardly seeing in the phloem of the branches of the basal part of the spike.
