摘要
目的：建立RP-HPLC法测定滋补参茸丸中补骨脂素和异补骨脂素含量的方法。方法：采用D ikma C18（5μm,150mm×4.6mm）色谱柱,流动相：乙腈-0.1%磷酸溶液（27：73,v/v）,检测波长246nm,流速：1.0m l.m in^-1。结果：补骨脂素在0.618×10^-3-12.36×10^-3mg.m l^-1（r=0.9998）、异补骨脂素0.598×10^-3 -11.96×10^-3mg.m l^-1（r=0.9999）之间呈良好的线性关系,补骨脂素的平均回收率为103.52%（n=6）,RSD为3.3%（n=6）;异补骨脂素的平均回收率为99.76%（n=6）,RSD为3.9%（n=6）。结论：使用RP-HPLC法测定滋补参茸丸中补骨脂素和异补骨脂素含量的方法简便、准确,可用于滋补参茸丸的质量控制。
Objective:To establish an RP-HPLC method for determination of Psoraden and Isopsoraden in Zibushenrong Pilulae.Methods: Dikma C18(5μm,150×4.6mm) was used.The mobile phase consisted of acetonitrile-0.1% H3PO4 and the determination wavelength at 246nm.the flow rate is 1.0ml/min.Results:The linear ranges of Psoraden and Isopsoraden were 6.18×10-3～123.6×10-3mg/ml(r=0.9998,RSD=3.6%) and 5.98×10-3～119.6×10-3 mg/ml(r=0.9999,RSD=4.2%).The average recoveries were 103.52%(n=6) and 99.76%(n=6).Conclusion: This method is simple and accurate,It is suitable for the quality control of Zibushenrong pilulae.
出处
《中医药信息》
2009年第1期66-67,共2页
Information on Traditional Chinese Medicine
基金
国家药典委员会药品标准提高行动计划项目
