摘要
目的检测人正常卵巢组织、卵巢浆液性囊腺瘤和浆液性囊腺癌组织中THY1蛋白的表达，探讨其表达变化与卵巢浆液性囊腺癌患者临床病理因素间的关系。方法应用免疫组化法，检测25例人正常卵巢组织、25例卵巢浆液件囊腺瘤组织和53例浆液性囊腺癌组织中THYI蛋白的表达。结果THY1蛋向在正常卵巢、卵巢浆液性囊腺瘤和浆液性囊腺癌组织中的阳性表达率分别为60．0％（15／25）、72．0％（18／25）和34．0％（18／53），IOD值分别为288449．2±60087．3、271655．6±66588．7和252087．6±45559．4。卵巢浆液性囊腺癌中，THY1蛋门的阳性表达率明显低于正常卵巢和卵巢浆液性囊腺瘤组织（P〈0．05），且THY1蛋白的表达景与卵巢浆液性囊腺痴的手术一病理分期、组织学分级及淋巴结转移旱显著负相关（均P〈0．05）。结论THY1基因的缺失町能与卵巢浆液性囊腺癌的发生、发展相关。
Objective To detect the cxpession of THY1 in ovarian serous cystadenocarcinoma tissues. Methods Immnunohistochemistry was performed to detect the expression of THY1 gene in formalinfixed, paraffin-embedded specimens of normal ovaries （ n = 25 ）, ovarian serous cystadenoma （ n= 25 ） , and serous cystadenocarclnoma （ n = 53 ）. The correlation of THYI expression with clinicopathological parameters was statistically analyzed. Results The positive expression rates of THY1 protein in normal ovaries, ovarian serous cystadenomas and ovarian serous cystadenocarcinomas were 60.0% （15/25）, 72.0% （18/25） and 34.0% （ 18/53 ） , respectively. The values of IOD of THY1 protein expression were 288 449.2 ± 60 087.3, 271 655.6 ± 66 588.7 and 252 087.6 ± 45 559.4, respectively. The expression of THY1 protein was significantly down-regulated in ovarian serous cystadenocarcinoma tissues compared with that in normal ovarian tissues and ovarian serous cystadenoma tissues （P 〈0.05 ）. THY1 expression was negatively correlated with surgical-pathological staging, histological differentiation and lymph node involvement （P 〈 0.05）. Conclusion The decreased level of THY1 expression may be related with the occurrence and development of ovarian serous cystadenocarcinoma.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2009年第2期118-120,共3页
Chinese Journal of Oncology
基金
2008年度广东省医学科研基金（A2008099）
