摘要
目的寻找一种非损伤性且能早期诊断ＳＬＥ并发狼疮性肾炎（ＬＮ）的指标。方法从肾组织中提取肾小球基底膜和肾小管上皮抗原，然后采用斑点ＥＬＩＳＡ（Ｄｏｔ－ＥＬＩＳＡ）法对５０例活动期ＳＬＥ患者血液中的肾小球基底膜抗体（ＧＢＭ－Ａｂ）及肾小管抗体（Ｔｕｂ－Ａｂ）进行检测。结果ＧＢＭ－Ａｂ检出阳性率达６８％，Ｔｕｂ－Ａｂ阳性率达７８％。与患者尿蛋白升高检出率（４８％）相比，两种抗肾抗体检出率均较高。此外，ＧＢＭ－Ａｂ或Ｔｕｂ－Ａｂ检测阳性的患者与临床诊断ＬＮ相符率达９２％。结论ＧＢＭ－Ａｂ或Ｔｕｂ－Ａｂ的检出可为临床早期诊断ＬＮ提供一定的依据。
Objective To isolate the glomerular basement membrane antigen (GBM-Ag) and tubular antigen (Tub-Ag) from fresh human kidney and to detect the corresponding antibodies in SLE patients for diagnosing lupus nethritis (LN).Methods The antigens were prepared according to literatures.Dot-ELISA was used to determin the GBM-Ab and Tub-Ab in 50 patients with diagnosed SLE active phase.Results The positive rates werer 68% for GBM-Ab and 78% for Tub-Ab,both were higher than 48%,the patients′ positive rate of proteinuria.The coincident rate between clinical and serological diagnoses for LN was 92%.Conclusion Detection of GMB-Ab or Tub-Ab can provide certain informative basis for establishing earlier diagnosis of LN in SLE patients.
出处
《徐州医学院学报》
CAS
1998年第2期97-99,共3页
Acta Academiae Medicinae Xuzhou
