摘要
在直链淀粉-三-(3,5-二甲基氨基甲酸酯)(Chiralpak AD-H)手性柱上对O,O-二甲基-1-(苯氧乙酰氧基)乙基膦酸酯和O,O-二甲基-1-(4-甲基苯氧乙酰氧基)乙基膦酸酯对映体的分离进行了研究。分别考察了流动相极性改良剂的类型和比例以及温度对拆分效果的影响,并初步讨论了拆分的热力学机理。实验结果显示:流动相为正已烷-异丙醇(体积比90∶10),流速为1.0mL/min,检测波长220nm,温度25℃,O,O-二甲基-1-(苯氧乙酰氧基)乙基膦酸酯和O,O-二甲基-1-(4-甲基苯氧乙酰氧基)乙基膦酸酯在Chiralpak AD-H柱上分离度Rs分别为2.63、1.56,对映体都可得到满意的基线分离。
The effect of chromatographic conditions including type and proportion of alcohol modifiers and column temperature to resoluting enantiomers of the O,O-dimethyl-1-（phenoxyacetoxy）ethylphosphonate and O,O-dimethyl-1-（4-methylphenoxyacetoxy）ethylphosphonate using amylose-tris-（3,5-dimethylphenylcarbamate） chiral stationary phase（chiralpak AD-H columns） on High-performance Liquid Chromatography were studied. Thermodynamical mechanism of enantio separation have also been preliminary discussed. In this study, baseline separation of the enantiomers of both analytes were obtained with a mobile phase of n-hexane/isopropanol（90：10, by vol） at a flow rate of 1.0 mL/min under 25℃ on the Chiralpak AD-H column.
出处
《农药》
CAS
北大核心
2009年第1期36-38,57,共4页
Agrochemicals
