摘要
目的建立一种稳定可重复的大鼠急性心肌梗死模型。方法SD大鼠经氯胺酮麻醉后,经口人工呼吸,开胸结扎左冠状动脉前降支。4周后行超声心动图、血流动力学和组织病理学检查。结果①心电图和组织病理学检查证实,成功建立了大鼠急性心肌梗死模型,梗死面积40%～45%(平均42%);②与假手术组比较,心肌梗死大鼠左室收缩末径、左室舒张末径和非梗死区增厚指数明显增加(P<0.01),左室后壁、左室前壁、梗死区变薄指数、左室射血分数和左室短轴缩短率显著降低(P<0.05,P<0.01);③心肌梗死大鼠动脉收缩压、舒张压、左室收缩压、左室内压最大上升和下降速率均低于假手术组(P<0.01),心率和左心室舒张末压高于假手术组(P<0.01);④两组大鼠左、右心室实际和相对重量以及胶原容积积分之间的差异有统计学意义(P<0.01)。结论本文建立心肌梗死动物模型的方法操作简单、重复性好、结果可信。
Objective To develop a steady and reproducible myocardial infarction（MI） model in rats. Methods SD rats were anaesthetized with ketamine. After linking with respiration machine, left anterior detending coronary artery was ligated. Echocardiogram, haemodynamies and histopathology were done four weeks after ligation. Results （1）The model of MI was established successfully and proved by electrocardiogram and histopathology. Infarct sizes were 40% - 45% （average 42% ）. （2）Compared with sham operation group, MI rats had higher left ventricular systolic diameter,left ventricular diastolic diameter and non-infarcted region thicken- ing index （P 〈0.01 ）, and lower posterior wall diameter, anterior wall diameter, infarcted region thinningz index, left ventricular ejection fraction and fractional shortening （P 〈 0. 05, P 〈 0. 01 ）. （3）Systolie blood pressure, diastolic blood pressure, left ventricular systolic pressure and the maximum rising and dropping rates of left ventrieular pressure decreased, while heart rate and left ventricular end-diastolic pressure increased after MI. （4） There were significant differences in left ventricular actual weight, right ventricular actual weight, left ventricular relative weight,right ventricular relative weight and collagen volume fraction between sham operation group and MI rats （ P 〈 0. 01 ）. Conclusion This experiment provided an easy way to establich the MI model, which was reproducible and credible.
出处
《中国实用医药》
2009年第2期1-3,共3页
China Practical Medicine
基金
广东省科技计划基金资助项目(2008B030301333)
关键词
冠状动脉
心肌梗死
模型动物
大鼠
Coronary artery
Myocardial infarction
Modelanimal
Rats
