摘要
目的建立稳定有效的转移性人肝癌裸小鼠原位种植合并肝动脉结扎模型。方法采用56只BALB／C-nu／nu裸小鼠，建立转移性人肝癌裸小鼠原位种植模型；随机分为2组，移植瘤术后2周进行肝动脉结扎（HAL）组和仅开腹为假手术组。两组中各随机抽出6只裸鼠，在2次手术后2d利用哌莫硝唑（Pimonidazole）作为乏氧探针行肝脏移植瘤标本免疫组织化学显色和Wester Rblot检测移植瘤组织中乏氧诱导因子（HIF）-1α蛋白的表达。结果人肝癌裸小鼠原位种植成瘤率为100．0％（56／56）；HAL手术成功率达93．3％（28／30）；与假手术组比较，HAL组移植瘤内乏氧细胞（Pimonidazole阳性细胞）显著增加（P〈0．05），移植瘤组织HIF-1α蛋白表达升高（P〈0．05）。结论转移性人肝癌裸小鼠原位种植合并肝动脉结扎模型手术成功率高，能够导致肝脏移植瘤有效乏氧，是一种较理想的研究肝动脉断流对人肝癌生物学特性影响的实验模型。
Objective To establish the metastatic model of human hepatocellular carcinoma （HCC） in nude mice via orthotopic transplantation and further to ligate hepatic artery so as to provide a practicable model for the study of biological effects of blocking of hepatic arterial blood. Methods Human HCC cell line MHCC97-L was inoculated into 56 BALB/c nude mice. All these cases were divided equally into HAL and sham-operation subgroups. Two days after the second operation, immunohistochemical staining and Western blot were carried out in 6 randomly selected nude mice of each subgroup to detect the expression of Pimonidazole and hypoxia inducible factor-1α （HIF-1 α）. Results All nude mice with xenograft tumors exhibited 100.0% （56/56） transplantability,and 93.3% （28/30） HAL operating successful rate was achieved. The expression levels of Pimonidazole and HIF-1 α were significantly higher in HAL group than in sham operation group （P 〈 0.05）. Conclusion After orthotopic tumor transplantation, HAL could reduce the arterial blood provision and consequently induce dramatic hypoxic effect and over-expression of HIF-1α. Such model was an appropriate measure to illuminate the biological effects of arterial blood shortage of human HCC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第1期115-117,共3页
Chinese Journal of Experimental Surgery
关键词
癌
肝细胞
原位移植
肝动脉结扎
模型
动物
Carcinoma, hepatocellular
Orthotopic implantation
Hepatic artery ligation
Model, animal
