摘要
目的了解精索静脉曲张(VC)及不明原因不育患者精子DNA碎片的发生比例。方法改进的精子染色质扩散(SCD)实验分析精子DNA碎片。检测VC不育患者39例,不明原因不育患者57例。以生育健康成年男性32例为对照组。结果VC不育患者SCD小光晕和无光晕精子(精子DNA碎片)比值平均为(36.6±18.9)%,VC不育组明显高于对照组(12.1±5.2)%(P<0.001),而大光晕和中光晕精子比值VC不育组明显低于对照组(P<0.01);不明原因不育患者精子DNA碎片比值平均为(26.8±10.2)%,与对照组[(12.1±5.2)%]比较有显著性差异(P<0.001)。结论SCD实验表明,VC及不明原因不育患者精子DNA碎片比值增高。
Objective： To understand levels of sperm DNA fragmentation in unexplained infertile males and infertile males with varicocele.
Methods： The level of DNA fragmentation was determined by improved sperm chromatin dispersion （SCD） test in unexplained infertile males （n=57） and infertile males with varicocele （n=39） and adult healthy fertile men （n=32） as control group.
Results. Thirty-nine cases with varicocele showed a significantly higher small halos and without halos EDNA fragmentation, mean average （36.6±18.9）%] in their spermatozoa compared to controls [（12.1±5.2）%] （P〈 0. 001）. The SCD patterns revealed a higher percentage （63.4±13.9）% of large size halos and medium-size halos for control group sperm than those obtained in the infertile males with varicocele [ （88. 0 ± 6.6）%] （P〈0. 01）. Mean percentage of spermatozoa with fragmented DNA in unexplained infertile males [（26.8 ± 10. 2）%] was significantly higher than that in the control [ （12.1 ± 5.2） % ] （P〈 0.001）.
Conclusion. The level of sperm fragmented DNA by SCD has an increase in unexplained infertile males and infertile males with varicocele.
出处
《生殖医学杂志》
CAS
2008年第6期465-468,共4页
Journal of Reproductive Medicine
基金
山东省人口和计划生育科研计划资助项目(鲁计育[2000-11])
