摘要
Objectives:To investigate the effect of sperm DNA fragmentation on outcomes of in vitro fertilization(IVF) and intracytoplasmic sperm injection(ICSI). Methods:A total of 242 cycles(154 IVF and 88 ICSI) from 235 couples were included.Sperm DNA fragmentation(SDF) and routine semen analysis were performed on the retrieval day.The rates of fertilization, embryo cleavage,good quality embryos,implantation and clinical pregnancy were measured. Results:Sperm DNA fragmentation index(DFI) in ICSI group was significantly higher than that in IVF group (P<0.01).The rates of fertilization,implantation and clinical pregnancy in ICSI were significantly higher than those in IVF with DFI≥24%(P<0.05).When DFI exceeded 24%,the OR for clinical pregnancy was 3.85(95% CI 1.40-10.59) comparing ICSI with IVF,and the OR for clinical pregnancy increased to 4.61(95%CI 1.09- 19.57) after inclusion of sperm concentration,progressively motile sperm percentage and female age as covariates. Conclusions:High DNA fragmentation might affect the outcome of ICSI and IVF.When DFI exceeds 24%, ICSI should be chosen instead of IVF.
Objectives： To investigate the effect of sperm DNA fragmentation on outcomes of in vitro fertilization （IVF） and intracytoplasmic sperm injection （ICSI）. Methods： A total of 242 cycles （154 IVF and 88 ICSI） from 235 couples were included. Sperm DNA fragmentation （SDF） and routine semen analysis were performed on the retrieval day. The rates of fertilization, embryo cleavage, good quality embryos, implantation and clinical pregnancy were measured. Results： Sperm DNA fragmentation index （DFI） in ICSI group was significantly higher than that in IVF group （P〈0.01）. The rates of fertilization, implantation and clinical pregnancy in ICSI were significantly higher than those in IVF with DFI≥24% （P〈0.05）. When DFI exceeded 24%, the OR for clinical pregnancy was 3.85 （95% CI 1.40-10.59） comparing ICSI with IVF, and the OR for clinical pregnancy increased to 4.61 （95% C1 1.09- 19.57） after inclusion of sperm concentration, progressively motile sperm percentage and female age as covariates. Conclusions： High DNA fragmentation might affect the outcome of ICSI and IVF. When DFI exceeds 24%, ICSI should be chosen instead of IVF.
出处
《生殖医学杂志》
CAS
2012年第B12期42-48,共7页
Journal of Reproductive Medicine
