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精子染色质扩散法检测精子DNA完整性方法探讨及临床意义 被引量：4

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摘要 目的探讨精子染色质扩散法检测精子DNA完整性这一实验方法的操作及其应用价值。方法对200例男性不育患者及50例健康对照者进行精液常规检查后,用染色质扩散法进行DNA完整性分析,观察实验条件的改变对检测结果的影响,从而为这一实验方法的应用和改进提供参考价值。结果 DNA完整性正常的精子染色质扩散而在核周形成晕环,并且晕环的宽度不小于精子头部的半径;而受损伤产生DNA碎片的精子则不形成晕环或晕环的宽度小于精子头部的半径。实验中,试剂、实验条件及操作方法的改变均影响检测结果的判读,从而为临床诊断带来一定困难。结论精子染色质扩散法对男性不育患者的病因诊断有较大临床意义,该实验过程中应掌握好实验条件及操作方法 。
作者 王玮玮 王厚照 张玲 刘芳
机构地区 中国人民解放军第一七四医院检验科
出处 《国际检验医学杂志》 CAS 2012年第14期1723-1724,共2页 International Journal of Laboratory Medicine
关键词 不育 男性 精子染色质扩散 精子 DNA
分类号 R698.2 [医药卫生—泌尿科学]
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参考文献12

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共引文献45

同被引文献57

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二级引证文献41

国际检验医学杂志
2012年 第14期

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