摘要
目的了解自、异体微粒皮混合移植促进创面愈合的机制。方法建立自、异体微粒皮混合移植大鼠模型。第1部分实验分为3组，每组9只大鼠：（1）自体皮组，移植面积扩张比为10：1的自体微粒皮；（2）混合1组，移植自、异体微粒皮，面积扩张比均为10：1；（3）混合2组，移植自、异体微粒皮，面积扩张比分别为10：1和10：3。第2部分实验亦分为3组，每组6只大鼠：（1）自体皮组，移植面积扩张比为20：1的自体微粒皮；（2）混合1组，移植自、异体微粒皮，面积扩张比分别为20：1、20：3；（3）混合2组，移植自、异体微粒皮，面积扩张比分别为20：1和20：6。术后从各组大鼠愈合创面取样，第1部分实验于术后2、3、4周取样，第2部分实验于术后3、4周取样，每组大鼠各时相点检测3个样本。行常规组织学与免疫组织化学染色，测定整合素p，的表达。显微镜下测量表皮层厚度。结果（1）HE染色显示，术后各组大鼠创面的表皮层厚度明显增加，真皮内有不同程度的血管扩张和单个核细胞浸润。（2）术后2～4周，第1部分实验中2个混合组大鼠皮肤表皮层均明显厚于自体皮组（P〈0．05或P〈0．01）；术后3、4周，第2部分实验中2个混合组大鼠皮肤表皮层厚度均明显厚于自体皮组（P〈0．05或P〈0．01）。（3）免疫组织化学染色显示，术后各组新生表皮中均可见整合素13．阳性细胞，以棘层和颗粒层为主。术后2周，第1部分实验中混合组整合素p。的阳性表达明显强于自体皮组（P〈0．01），且混合1组的表达（10982±2169）明显强于混合2组（4240±512，P〈0．01）；术后3～4周，混合1组的表达仍明显强于自体皮组和混合2组（P〈0．01）。第2部分实验仅在术后3周时混合2组整合素p，的阳性表达（1618±171）明显高于自体皮组（1060±146，P〈0．05）。结论自、异体微粒皮混合移植中，整合素β1的异位表达和表达增强与表皮细胞的增殖分化、创面再上皮化以及表皮层增厚有密切关系，在促进创面愈合过程中，整合素β1，阳性表达细胞很有可能发挥了重要作用。
Objective To study the mechanism of promoting wound healing with mixed grafting of autologous and allogeneic microskin in rats. Methods Fifteen male Wistar rats served as alloskin donor . Forty-five female SD rats with full-thickness skin defect served as recipients in the study. In part one experiment,27 SD rats were randomly divided into group I （n=9, without allogeneic microskin）, group Ⅱ （n = 9,with mixed grafting of allogeneic microskin at area expansion rate of 10：1 ） ; group Ⅲ（n=9, with mixed grafting of allogeneic microskin at area expansion rate of 10： 3） with grafting with the same amount of autologous microskin at area expansion rate of 10：1. In part two experiment, 18 SD rats were also divided into group I （n=6, with autologous microskin only） ; group Ⅱ （n=6, with mixed grafting of autologous and allo- geneic microskin with area expansion rate of 20：1 and 20：3 respectively） ; group Ⅲ （ n = 6, with mixed grafting of autologous and allogeneic microskin with area expansion rate of 20：1 and 20： 6,respectively）. Biopsy samples were obtained from healed wound area of SD rats in each group at different time points after operation. The histological changes, epidermal thickness, and immunohistochemical staining of Integrinβ were observed. Results （1） HE staining showed the thickness of epidermis in each group increased obviously, and various amounts of mononuclear cell infiltration and different degrees of vasodilation appeared in the dermal laver during 2-4 weeks. （2）Epidermal thickness in group Ⅱ and Ⅲ of part one experiment were significantly thicker than that in group I during 2 - 4 weeks after operation （ P 〈 0.05） , and the similar result was also seen in part two experiment on 3 and 4 weeks after operation （ P 〈 0.05）. （3） A positive staining pattern for Integrinβ1 was seen in the suprabasal layers（ especially in the spinous and granular layers） in all groups. In part one experiment, the expression of Integrinβ1 in group Ⅱ and Ⅲ were obviously higher than that in group I on 2 week after operation （ P〈0.01） ,and the expression of Integrinβ1 in group Ⅱ（10 982 ± 2169） was also higher than that in group Ⅲ （4240±512, P〈0.01）; the expression of Integrinβ1 in group Ⅱ was still higher than that in group Ⅰ and Ⅲ （P〈0.01 ） 3 and 4 weeks after operation. In part two experiment, the expression of Integrinβ1 in group Ⅲ （1618±171 ） was higher than that in group Ⅰ 3 weeks after operation （1060±146, P 〈 0.05 ）. Conclusion The ectopic and increased expression of Integrinβ1 was closely associated with the proliferation and differentiation of epidermal cells, wound reepithelialization and thickened epidermis in mixed grafting of autologous and allogeueic microskin. Integrin.Integrinβ1 may be responsible in promoting wound healing.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2008年第6期445-449,共5页
Chinese Journal of Burns
基金
国家重点基础研究发展计划（2005CB522605）
国家高技术研究发展计划（2006AA02A121）
