摘要
以西洋杜鹃的嫩叶片为外植体,研究了其离体培养和植株再生的过程。结果表明:叶片愈伤组织诱导最佳培养基为Read+ZT 5.0mg/L,可用回归模型y=19.21+7.32x_1来估测西洋杜鹃叶片愈伤组织诱导情况。叶片愈伤组织增殖培养基同初代培养基,继代30 d后体积可增为原来的3倍,重量增为原来的2.5～4.5倍。愈伤组织分化的最佳培养基配方为:Read+ZT 1.0 mg/L+NAA 0.01 mg/L,分化率为87.5%,分化出苗数在12～18株。生根培养基以Read+IBA 1.0 mg/L+NAA2.0 mg/L+ZT0.2 mg/L为好,生根率达90%以上。炼苗基质选腐殖土:黄沙:珍珠岩=5:1:1为宜,存活率达88.33%。
The technique system of induction of callus from leaf explants of Rhododendron hybridum and its regeneration were established. The results showed that Read ＋ ZT 5.0 mg/L was the best medium to induce callus from leaf explants. And Linear regression modely = 19.21 ＋ 7.32 x1 could estimate the growth condition of callus. Multiplication medium was the same as induction medium of callus. After 30 d inoculated, callus reached 3 times of its volume,2.5 -4.5 times of its weight. Read ＋ ZT 1. 0 mg/L ＋ NAA 0.10 mg/L was the best medium inducing callus to differentiate shoots and a high frequency could reach 87.5%. The regenerated shoots rooted readily on Read medium plus 1.0 mg/L IBA 2.0 mg/L NAA and ZT 0.2 mg/L. The rate of roofing could reach 90%. The regenerate plants were acclimatized the soil of muck, yellow sand and perlite （5： 1： 1） ,the survival rate of plantlets was 88.33%.
出处
《种子》
CSCD
北大核心
2008年第11期46-49,共4页
Seed
基金
国家农业成果转化项目和贵州省攻关项目资助
关键词
西洋杜鹃
叶片
离体培养
植株再生
Rhododendron hybridum
leaf
inoculating in vitro
plant regeneration
