摘要
目的探讨核因子-κB(NF-κB)p65蛋白在大肠癌组织中表达与细胞增殖之间的关系。方法采用免疫组织化学方法,检测32例大肠癌组织和30例癌旁组织中NF-κB p65蛋白的表达;用增殖细胞核抗原(PCNA)指数测定、分析其与NF-κB p65之间的关系。结果NF-κB在大肠癌组织中的阳性表达率为67.8%,30例癌旁组织中无1例阳性表达,两者间阳性率比较差异有显著性(P<0.01)。32例大肠癌组织PCNA指数为20.2%～78.5%,在高、中、低不同分化肿瘤组织中的表达差异有显著性,在淋巴结阳性组与阴性组之间阳性率比较,差异有显著性(P<0.01)。结论NF-κB p65蛋白与大肠癌的发生有关,NF-κB p65蛋白可能通过上调PCNA对大肠癌细胞增殖起重要作用。
Objective To investigate the relationship between the expression of nuclear factor kappa B（NF-κB） p65 and tumor cell proliferation in carcinoma of large intestine. Methods Immunohistochemistry method was performed to detect the expression of NF-κB p65 in 32 cases of carcinoma of large intestine and 30 cases of tumor-adjacent tissues. Proliferating cell nuclear antigen （PCNA） index was used to investigate its relationship with the expression of NF-κB p65. Results The positive expression rate of NF-κB p65 was 67.8% in carcinoma of large intestine and it was negative in 30 cases of tumor-adjacent tissues. The positive expression rate of NF-κB p65 in carcinoma of large intestine was significantly higher than in tumor-adjacent tissues （P 〈0.01）. Overexpression of NF-κB p65 in carcinoma of large intestine was related to the lymph node metastasis （P 〈0.01）. PCNA index was 20.2%-78.5% in carcinoma of large intestine and significantly different with the degree of differentiation. Conclusion NF-κB p65 may play an important role in the development of the carcinoma of large intestine and is correlated with neovascularization and tumor cell proliferation.
出处
《兰州大学学报（医学版）》
CAS
2008年第4期4-7,共4页
Journal of Lanzhou University（Medical Sciences）
关键词
核因子-ΚB
P65
增殖细胞核抗原
大肠癌
免疫组织化学方法
nuclear factor kappa B p65
proliferating cell nuclear antigen
carcinoma of largeintestine
immunohistochemistry method
