摘要
人参原生质体培养未见报道成功。Harn(1974)曾用人参幼叶,幼根和上胚轴进行原生质体分离,但得到的数量很少,无法进行培养。本实验以人参培养细胞为材料,通过培养再生了愈伤组织。
Protoplasts were enzymatically isolated from the cultured cell line No.246 of Panax ginseng and suspended in a 67V-D liquid medium.After 35 days of culture, the cell clusters derived from protoplasts were transfered to a 67V-E semisolid medium.When the cell clusters grew to 2mm approximately,they were transfered to common 67V medium for the callus regeneration.
关键词
人参
原生质体培养
愈伤组织
再生
Panax ginseng
Protoplast culture
Callus regeneration
