摘要
目的研究慢性不可预见性应激对小鼠行为学的损害及L-酪氨酸的保护作用。方法采用慢性不可预见性应激加孤养结合的方法建立慢性不可预见性应激动物模型，在此基础上采用自发运动实验和Morris水迷宫实验对小鼠行为学进行测试，测定脑组织中去甲肾上腺素（NE）、多巴胺（DA）含量进一步评价慢性不可预见性应激对中枢神经系统的损害及药物干预效果。结果与正常对照组相比，应激组小鼠对新环境的探究行为和行走路程从第3周开始明显减少，至第4周[（1．36±0．43）m]与正常对照组[（2．01±0．31）m]相比差异有显著性（P〈0．01），补充L-酪氨酸后自发运动成绩[（1．93±0．37）m]明显提高（P〈0．01）；Morris水迷宫实验结果显示：正常对照组逃避潜伏期[（31．5±17．6）s]逐渐缩短，而应激组[（62．4±25）s]则与之相反并且差异显著（P〈0．05）；L-酪氨酸组小鼠逃避潜伏期[（32．3±21）s]亦逐渐缩短且与应激组差异明显（P〈0．05），应激组皮层、海马和下丘脑的DA含量均显著降低（P〈0．05），应激组皮层、下丘脑NE含量也显著降低（均P〈0．01）：补充L-酪氨酸后小鼠大脑皮层、海马和下丘脑组织中的DA、NE含量均升高。结论慢性不可预见性应激小鼠出现体力下降、抑郁情绪倾向及空间学习记忆能力降低等行为学改变，应激小鼠脑组织DA、NE含量明显减少。
Objective Using a chronic unpredictable stress animal model to study the changes of the learning and memory of mice and effects of L-tyrosine interference were observed. Methods To create a chronic unpredictable stress animal model, spontaneous actions test and Morris water maze test were be used to study the changes of the learning and memory of mice. And at last, the content measurements of NE, DA in brain were employed to further estimate the impairment of stress and the effect of drug interference. Results Spontaneous actions indueed by chronic unpredictable stress were remarkably reduced [ （1.36±0.43 ）m vs （2.01±0.31 ）m, P 〈0.01 ] and being supplied with L-tyrosine[ （ 1.93±0.37）m vs （ 1.36±0.43 ）m, P〈0.011 ,and morris water maze test showed that the period of the stress group（62.4±25 ） s had been gradually delayed（P〈 0.05 ）. With the supplement of L-tyrosine,it had got an elevation （P 〈 0.05, respectively）. The contents of DA in pallium, hippocampi and hypothalamus of the mice submitted to stress alone were significantly decreased （ respectively, P 〈 0.01, P 〈 0.01, P 〈 0.05 ） and so were the level of NE（P 〈 0.01 ） except of hippoeampi, both of whieh would be reversed with the supplement of L-tyrosine. Conclusion Chronic Stress could change the behaviors of mice ob- servably and the contents of DA, NE in pallium,hippocampi and hypothalamus.
出处
《中国行为医学科学》
CSCD
2008年第11期976-978,共3页
Chinese Journal of Behavioral Medical Science
