摘要
目的:研究慢性不可预见性应激(CUS)和氟西汀(Flu)治疗对雌鼠类抑郁样和焦虑样行为的影响。方法:成年雌性SD大鼠随机分为NS组、Flu组、CUS+NS组和CUS+Flu组,每组6只。CUS+NS和CUS+Flu组每日CUS前30 min分别注射2.5 mL/kg NS或10 mg/kg Flu;NS和Flu组仅给予相同剂量的NS或Flu。最后一次应激注射后第2 d用糖水消耗实验和强迫游泳评估类抑郁行为,开场试验评估类焦虑行为。结果:糖水消耗实验示糖水消耗百分比无组间差异,但CUS+NS组糖水消耗量明显低于其它3组;强迫游泳示CUS+NS组的不动时间显著长于其它各组,而NS、Flu和CUS+Flu组间无统计学意义;开场实验示CUS和CUS+Flu组中央/总路程低于NS和Flu组(P<0.05),CUS+NS组的中央活动时间少于Flu组,CUS+Flu组的中央活动时间少于NS和Flu组。结论:CUS大鼠具有类抑郁样行为,同时伴类焦虑样行为,Flu治疗可以逆转类抑郁样行为,但对类焦虑样行为无效;Flu对正常大鼠无情绪改变作用。
Objective： To study the effects of chronic unpredictable stress（CUS） and fluoxetine（ Flu） treatment on depressive-like and anxlety-like behaviors of adult female rats. Methods ： Twenty-four virgin fern.ale rats were assigned evenly to normal sodium（NS） group, fluoxetine（Flu） group, chronic unpredictable stress（CUS） exposed plus NS injection group（CUS＋NS）, and CUS exposed plus Flu treatement group（CUS ＋Flu）. CUS ＋NS group and CUS ＋Flu group received a daily intraperitoneal injection of 2.5 mL/kg NS or 10 mg/kg Flu 30 minutes before CUS. NS group and Flu group only received a daily intraperitoneal injection of 2.5 mL/kg NS or 10 mg/kg Flu. The second day after the last in- jection, the sucrose consumption test and forced swim test were used to test the depressive-like behavior and the open field test was used to test anxiety-like behaviors. Results： There was no difference in sucrose consumption percentage, but the sucrose intake had significant difference among groups, with CUS ＋NS groups consuming less sucrose than all other groups. The immobility time had significant difference in 5-min swim test among groups, with CUS ＋NS group longer than the other three groups, and there was no difference among NS, Flu and CUS ＋flu groups. The central-to-total distance traveled ratio in the CUS group and the CUS ＋Flu group was lower than that in the NS group and Flu group, and the sum time in central part in the CUS ＋NS group was less than that in the Flu group, and in the CUS ＋Flu group was less than that in the NS group and the Flu group. Conclusion： CUS induces depressive-like and anxiety-like behavior, and flu treatment may reverse the depressive-like but not the anxiety-like behavior. Flu is devoid of mood-changing effects in normal rats.
出处
《汕头大学医学院学报》
2010年第3期145-148,共4页
Journal of Shantou University Medical College
关键词
慢性不可预见性应激
氟西汀
类抑郁样行为
类焦虑样行为
chronic unpredictable stress, fluoxetine
depressive-like behavior, anxiety-like behavior
